Table of Content

28 February 2010, Volume 5 Issue 1

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A case report of sinusitis due to Scedosporium prolificans:identification of causative agent and antifungal susceptibility testing in vitro

Paride Abliz, Takashi Yaguchi, LUO De-mei, Yan Hui, Katsuhiro Kamei, Kazuko Nishimura

2010, 5(1):  1-4. 

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Objective To report the first case of sinusitis caused by Scedosporium prolificans in China. To identify the causative agent and to study its untifungal succeptibility in vitro.Methods Maxillary excretion was drawn for cultivation and morphological identification. β-tublin and rDNA were isolated for ITS sequence analysis. Antifungal susceptibility was determined for 7 kinds of drug in vitro.Methods Scedosporium prolificans was identified by morphological characteristics and sequences data.Scedosporium prolificans was identified by morphological characteristics and sequences analysis.Conclusions Mycosis caused by Scedosporium prolificans is rare,identification of which is based on morphological characteristics and sequence analysis. This strain is resistant to many untifungal agents.

Variability analysis of Trichophyton rubrum isolates in members of the same family

HE Xiao-dan, RAN Yu-ping, DAI Ya-ling, QING Chun, ZHANG Hao, XIANG Yun

2010, 5(1):  5-8,21. 

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Objective To compare the cariability of the Trichophyton rubrum strains isolated from different members by PCR in the same family and to determine the dermatophytosis were transmitted within the family or infected from outside.Methods Scales scraped from patients with dermatophytosis in the same family were inoculated on SDA.T.rubrum was identified by physiological and morphological characteristics.If strains from different patients were found to belong to the same species, RAPD and specific amplification of tandem repeat sub-elements (TRSS) in strain typing were performed and the products were compared.Methods All the strains isolated from 16 patients in 8 families (2 patients per family) were identified as T.rubrum by morphological methods. In 4 families, distinction was identified by RAPD with 4 arbitrary primers. In 5 families the isolates from each family were found to be different in the types of fingerprints produced by amplifying the TRS-1 region specifically. Fingerprints of TRS-2 amplified from isolates differred in 2 families. In all differences were found in strains from 6 families.Conclusions Dermatophytosis in one family is more frequently infected from outside than inside. Fingerprints produced by specifically amplifying TRS-1 are more effective in distinguishing strains of T.rubrum spp than RAPD, and TRS-1 is more suitable for strain typing in T.rubrum.RAPD combimed with TRSS may distinguish T.rubrum strains to maximum.

Identification of prototheca by phenotype and molecular biology techniques

ZHANG Qiang-qiang, ZHAO Ying

2010, 5(1):  9-12,25. 

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Objective To identify Prototheca and its varieties by phenotype and molecular biology techniques.Methods The structure and phenotypic characteristics, physiology, biochemistry and molecular biological identification, sensitivity to common anti-fungal drugs and characteristics of histopathology were reviewed,summarized and analyzed.Methods Two strains of Prototheca (P.zopfii var.hydrocarbonea and P.zopfii var .portoricensis) were identified from cerebrospinal fluid and lymph nodes by phenotypic and molecular biology techniques and relevent photographs were showed.Conclusions Diagnosis of Protothecosis mainly depends on mycological examinations including direct microscopic examination,culture,and histopathology because of no characteristic in clinical.Carbohydrates assimilation, temperature resistance and molecular biological methods would be helpful for strain identification.

Influence of HOG1 gene on ultrastructure of Candida albicans

HUANG Yue, ZHANG Zi-ping, CHENG Bo

2010, 5(1):  13-16. 

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Objective To explore the influence of HOG1 gene on ultrastructure of Candida albicans.Methods Ultrastructure of Candida albicans in HOG21 group (hog1/hog1 double mutant) and WT group (wild type) were observed under scanning electron microscope (SEM) and transmission electron microscope (TEM).Result Under scanning electron microscope, two groups of cells appeared round or ellipse with multilateral buds growing.The HOG1 gene mutant were rough and uneven with less numbers of buds,while WT cells appeared smooth with much more petal-like budding scars.Under transmission electron microscope,the walls of HOG1 gene mutant cells were incomplete while the structure of wild type cell’s wall was integral. The cytoplasmic membrane of HOG1 gene mutant cell changed in the presence of evaginations ,discontinuity,budding of small vesicles accumulated around the cell membrane.Conclusions HOG1 gene may paly a role in the structure of cell’s wall of Candida albicans.

Expression and the clinical significance of proteases MEP and SUB in Trichophyton rubrum

CHEN Rong, JIANG Pei-hong

2010, 5(1):  17-21. 

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Objective To explore the expression and clinical significance of gene MEP and SUB in Trichophyton rubrum.Methods Total RNA of Trichophyton rubrum was extracted for semi-quantitative RT-PCR to detect expression changes of metalloproteinases (MEP) and subtilisins (SUB).Methods Trichophyton rubrum SUB expression was closely related to clinical symptoms,but irrelevant to age, gender,or disease duration. The expression levels of MEP varied to age, gender, disease duration and clinical classification,without significant differences.Conclusions The virulence of Trichophyton rubrum may be related to the different expression of gene SUB.

A case of Graves ophthalmopathy accompanied by corneal fungus infection

ZHANG Lan-yu, ZHENG Jiao-yang, FENG Xiao-yun, ZOU Jun-jie, LIU Zhi-min

2010, 5(1):  26-28. 

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To report a case of Graves ophthalmopathy with fungus infection in cornea.An 31 year old woman was suffered from hyperthyroidism with infiltrating exophthalmos,which led to reduced visual acuity and macular edema.Antithyroid drugs (ATD) were firstty administered,but hyperthyroidism did not improve after one-month treatment.High Dose Methylprednisolone and local injection of triamcinolone acetonide were then applied for three months while scanttered ulcer of cornea and light perception missing appeared.One week later,the patient was suffered from high fever,the highest of 39.5℃,fluctuating from 36.7℃ to 37.3℃ after anti-bacteria treatments.Hyphae and spores were found in the corneal secretion.Temperature and visual acuity recovered and the area of corneal ulcer reduced after combinition therapy of ATD,nutrition support and antifungal measures.