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    Guidelinesforthe diagnosis andtreatment oftinea corporis andtinea crurisin China
    The Working Group on Guidelinesforthe Diagnosis and Treatment of Tinea corporis and Tinea Crurisin China
    Chinese Journal of Mycology    2023, 18 (3): 193-197.  
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    Chinese Journal of Mycology    2022, 17 (3): 177-182.  
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    Chinese Journal of Mycology    2023, 18 (3): 265-269.  
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    Chinese Journal of Mycology    2022, 17 (4): 319-323.  
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    Chinese Journal of Mycology    2022, 17 (4): 349-352.  
    Abstract46)      PDF (814KB)(189)       Save
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    Chinese Journal of Mycology    2023, 18 (1): 58-64.  
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    Meta-analysis of trimethoprim-sulfamethoxazole combined with caspofungin in the treatment of Pneumocystis jirovecii pneumonia
    DUNA Yan, XU Yuan, YANG Changqing, ZHANG Jinping
    Chinese Journal of Mycology    2023, 18 (1): 1-7.  
    Abstract167)      PDF (2630KB)(160)       Save
    Objective To systematically evaluate the efficacy and safety of trimethoprim-sulfamethoxazole (TMP/SMZ) combined with caspofungin in the treatment of Pneumocystis jirovecii pneumonia (PJP). Methods PubMed, Embase, Cochrane library, CBM, CNKI, Wanfang, VIP were searched for clinical randomized controlled trials of TMP/SMZ combined with caspofungin in the treatment of PJP, and Meta-analysis was performed. The outcome indicators were total effective rate, incidence of adverse reactions Statistical analysis was performed using RevMan 5.4 software. Results A total of 20 studies were included, involving 1 802 PJP patients. Meta-analysis results showed that compared with TMP/SMZ monotherapy, the effective rate of TMP/SMZ combined with caspofungin in the treatment of PJP was significantly improved, [RR =1.29, 95% CI (1.22,1.37),P< 0.00001], and no Increase the incidence of adverse drug reactions [RR =0.91,95% CI(0.79, 1.05),P=0.21>0.05]. Conclusion The effect of TMP/SMZ combined with caspofungin in the treatment of PJP is better than that of TMP/SMZ monotherapy, without increasing adverse drug reactions, and it is recommended for clinical use.
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    Chinese Journal of Mycology    2022, 17 (4): 339-348.  
    Abstract37)      PDF (1000KB)(157)       Save
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    Retrospective analysis of Kodamaea ohmeri infection in Asia from 2002 to 2022
    ZHU Xinlin, LI Chen, HU Dongying, CHEN Xianzhen, JIANG Weiwei, LIU Yinuo, CHEN Tianyang, CHEN Tiancheng, LIAO Wanqing, LIU Xiaogang, PAN Weihua
    Chinese Journal of Mycology    2023, 18 (2): 123-129.  
    Abstract84)      PDF (849KB)(150)       Save
    Objective To analyze epidemiological trends and clinical characteristics of cases caused by Kodamaea ohmeri in Asia, and to provide a scientific basis for improving the clinical diagnosis and treatment of K. ohmeri infections. Methods Using the methods of literature review and meta-analysis to analyze retrospectively the information of patient’s nationality, sex and aged composition, risk factors, onset organs, clinical manifestations, therapeutic methods and prognosis of K. ohmeri. Results K. ohmeri infections could occur in both immunocompetent and immunocompromised person. The gender ratio of the infection host was 1.59:1, predominantly in premature neonate and middle aged people, and the blood system was the most commonly affected site. More than half of the patients had immunodeficiency diseases or other risk factors, and there were no early specific symptom with chief complaints of fever. Diagnosis of K. ohmeri infections mainly relied on culture. The patients had a poor prognosis, with high mortality. Conclusion The incidence of K. ohmeri infections has increased recently and the diagnosis mainly depends on culture. Strengthening the in vitro antifungal susceptibility monitoring and the studying of epidemiology for K. ohmeri are significant to improve the diagnosis and prognosis of K. ohmeri infections.
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    Chinese Journal of Mycology    2022, 17 (4): 335-338.  
    Abstract52)      PDF (825KB)(145)       Save
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    Chinese Journal of Mycology    2022, 17 (3): 247-250.  
    Abstract41)      PDF (1179KB)(145)       Save
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    Chinese Journal of Mycology    2023, 18 (1): 38-41.  
    Abstract113)      PDF (813KB)(134)       Save
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    Chinese Journal of Mycology    2023, 18 (2): 178-182.  
    Abstract67)      PDF (809KB)(125)       Save
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    Efficacy and safety of Meifute® antifungal solution combined with Feijia® antifungal solution in the treatment of onychomycosis
    XU Xiaoguang, YIN Jiankai, LIU Zhu, ZHENG Rui, LIU Weida, WEN Hai
    Chinese Journal of Mycology    2023, 18 (1): 42-46.  
    Abstract398)      PDF (1256KB)(121)       Save
    Objective To evaluate the efficacy and safety of Meifute® antifungal solution combined with Feijia® antifungal solution in the treatment of onychomycosis. Methods From December 2020 to November 2022, 120 patients were enrolled in Fumei Outpatient Departments for this study. First of all, a single-session soak in Meifute® antifungal solution was used to treat tinea pedis; and then, diseased nails were treated with Feijia® antifungal solution once a week for 28 weeks routinely; finally, another time of Meifute® antifungal solution treatment was given to consolidate the effect. Efficacy and safety evaluation was performed after treatment, and a questionnaire survey of treatment satisfaction was carried out after the completion of the treatment course. Results Finally 112 patients completed the study and 588 nails were included. Efficacy and satisfaction:overall effective rate was 58.0% (341/588), the cure rate was 20.6% (121/588), and the comprehensive satisfactory rate was 93.8% (105/112). The efficacy of the first toenail (52.9%) was lower than that of other toenails (60.7%). And there was no significant difference in the clinical response rate between DLSO (56.6%) and TDO (61.4%). Safety:The adverse reactions were all local symptoms, such as subungual hemorrhage (2 cases), exfoliation of epidermis around nails (4 cases), mild redness and swelling of skin (6 cases), mild to moderate burning or tingling (16 cases) after soak. All patients could tolerate the process without serious adverse reactions. Conclusion The use of Meifute® antifungal solution combined with Feijia® antifungal solution is simple, effective, safe, and highly compliant in the treatment of onychomycosis, so it can be regarded as a clinical alternative treatment for onychomycosis.
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    Chinese Journal of Mycology    2023, 18 (1): 90-96.  
    Abstract100)      PDF (917KB)(120)       Save
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    Chinese Journal of Mycology    2022, 17 (3): 251-254.  
    Abstract38)      PDF (960KB)(115)       Save
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    Chinese Journal of Mycology    2023, 18 (1): 80-85.  
    Abstract81)      PDF (1217KB)(108)       Save
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    Chinese Journal of Mycology    2022, 17 (3): 260-264.  
    Abstract35)      PDF (982KB)(103)       Save
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    Chinese Journal of Mycology    2022, 17 (4): 324-329.  
    Abstract40)      PDF (868KB)(94)       Save
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    Chinese Journal of Mycology    2022, 17 (3): 231-234.  
    Abstract29)      PDF (951KB)(92)       Save
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    Chinese Journal of Mycology    2023, 18 (1): 53-57.  
    Abstract81)      PDF (853KB)(89)       Save
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    Analysis of clinical characteristics and risk factors of nosocomial fungal urinary tract infection in ICU
    ZHU Junfeng, ZHOU Ziyang, HU Wangchao, TANG Jianguo
    Chinese Journal of Mycology    2022, 17 (4): 273-277.  
    Abstract44)      PDF (1043KB)(88)       Save
    Objective To investigate the clinical characteristics and risk factors of nosocomial fungal urinary tract infection in ICU. Methods The clinical data of patients with fungal urinary tract infection in the ICU of Shanghai Fifth People's Hospital affiliated to Fudan University from January 2016 to December 2020 were retrospectively analyzed, and the clinical characteristics and risk factors of the patients were described. Results ①From 2016 to 2020, a total of 2557 patients were enrolled in the intensive care unit of Shanghai Fifth People's Hospital Affiliated to Fudan University, and 130 patients met the research conditions. The incidence of fungal urinary tract infection was 5.1%. ②The clinical manifestations of nosocomial fungal urinary tract infection in ICU were atypical, and its occurrence was related to gender, age, and catheter indwelling time, but not to the year.③In the urine culture, Candida albicans was the main pathogen, accounting for 42.5%, followed by Candida glabra (31.3%), Candida tropicalis (19.4), Candida parapsilosis (4.5%), and other Candida (2.2%).④The risk factors of nosocomial fungal urinary tract infection in ICU were analyzed. Univariate analysis showed that diabetes, primary disease stroke, antibiotics, preinfection white blood cell count and preinfection enema had statistical significance (P<0.05).The significant indicators from univariate analysis were incorporated into the multivariate analysis. The Results showed that diabetes mellitus (P<0.001,OR=5.488), primary disease stroke (P=0.009,OR=2.782), preinfection white blood cell count (P=0.014,OR=3.515) and preinfection enema (P=0.001,OR=3.567) were independent risk factors for nosocomial fungal urinary tract infection in ICU. Conclusion For the nosocomial fungal urinary tract infection in ICU, we should take active measures to treat the primary disease and the basic disease, and at the same time, we should be alert to the enterogenous pollution caused by enema.
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    Chinese Journal of Mycology    2023, 18 (3): 285-288.  
    Abstract71)      PDF (838KB)(88)       Save
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    Chinese Journal of Mycology    2023, 18 (1): 75-79.  
    Abstract70)      PDF (840KB)(86)       Save
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    Chinese Journal of Mycology    2023, 18 (3): 270-272,281.  
    Abstract45)      PDF (905KB)(85)       Save
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    Distribution of pathogenic yeast species of vulvovaginitis and its epidemiological characteristics
    ZHOU Yihua, DENG Shuwen, ZHANG Lu, ZHANG Hong, ZHAO Rongfen, WANG Yufeng, SUN Yanxia, ZHU Hui
    Chinese Journal of Mycology    2023, 18 (1): 12-18.  
    Abstract140)      PDF (1033KB)(80)       Save
    Objective To investigate the epidemiological characteristics of vulvovaginitis caused by different yeast spp. Methods Totally 1 083 yeast isolates were collected from patients with vulvovaginal candidiasis (VVC). These isolates were identified to the species level according to the genes of 26S rRNA D1/D2 domain. The clinical characteristics VVC cases caused by these different species were analyzed. Results The results showed that Candida albicans remain the prodominent species(71.38%) causing VVC, while Non-candida albicans (NAC) account for 28.62% among which C.glabrata account for 19.02%. The risk factors included 20-40 years of age, being married,and pregnancy. NACs were more often isolated in pregnant women. The incidence of recurrent vaginal candidiasis caused by non-albicans Candida were higher. Conclusion Routine culture of secretion from VVC patients, molecular identification to species level of VVC causative agents, and identification of non-albicans Candida species would help clinicians to explore an effective therapy for VVC.
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    Study on the antifungal activity of small G protein inhibitor CASIN in vitro
    CHAO Wen, DENG Zhongyu, GUO Shijin, GUO Yifan, YAN Lan, QIU Lijuan, LV Quanzhen
    Chinese Journal of Mycology    2022, 17 (4): 289-293.  
    Abstract41)      PDF (1504KB)(78)       Save
    Objective In order to find antifungal compounds with new structure, the antifungal activity of small G protein inhibitors were tested in vitro. Methods Micro-dilution method was used to investigate the minimum inhibitory concentration (MIC) of different small G protein inhibitors against Candida. After that, the more active compounds were selected for the further antifungal spectrum determination. Time-growth curves and time-kill curves were used to investigate the effect of CASIN on the growth and proliferation of Candida albicans. Hyphae formation experiment was used to investigate the inhibitory effect of compound CASIN on the formation of Candida albicans hyphae. XTT was used to investigate the inhibitory effect of compound CASIN on the formation of Candida albicans biofilms. Results Among the investigated small G protein inhibitors, CASIN had the strongest in vitro antifungal activity. It also had a good inhibitory effect on Candida parapsilosis, Candida krusei, Candida tropicalis, and Candida glabrata with the MIC of 8-16 μg/mL. 16 μg/mL of CASIN could effectively inhibit the growth and proliferation of Candida albicans in YPD medium, and also exhibited fungicidal activity against Candida albicans in RPMI1640 medium. The hyphae formation of Candida albicans was significantly inhibited by 8 μg/mL of CASIN. 8 μg/mL of CASIN had an inhibition rate of more than 80% for the biofilms formation of Candida albicans. Conclusion CASIN, a small G protein inhibitor, has strong antifungal activity in vitro.
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    Chinese Journal of Mycology    2022, 17 (4): 330-334.  
    Abstract43)      PDF (852KB)(77)       Save
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    Effect of terbinafine hydrochloride cream combined with 3% boric acid alcohol ear drops on fungal otitis externa
    DU Yucong, LIU Huanhai
    Chinese Journal of Mycology    2022, 17 (3): 227-230.  
    Abstract35)      PDF (925KB)(76)       Save
    Objective To explore the clinical efficacy of THC combined with 3% baa ear drops in the treatment of mycosis. Methods A total of 144 patients with external auditory canal mycosis treated in our hospital were enrolled. The patients were randomly divided into group A and group B. Group A was treated with 3% BAA and THC, and group B was treated with BAA alone. The therapeutic effects and fungal culture results of the two groups were compared and analyzed. Results The cure rate and clinical efficacy score of group A were significantly higher than those of group B (P<0.05); The improvement of pruritus, otorrhea and tinnitus in group A was more obvious than that in group B (P<0.05); Aspergillus and Candida accounted for 57.7% and 22.5% in the positive fungal culture, and they were highly sensitive to terbinafine. Conclusion THC combined with 3% baa ear drops is an effective method for the treatment of fungal otitis externa.
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    Experimental study on the in vitro antibacterial activity of Medi hair spray dressing against Malassezia
    GAO Ruijia, LIU Shirui, SUN Jinpeng, FAN Jinghua, LI Fuqiu
    Chinese Journal of Mycology    2023, 18 (1): 19-23,41.  
    Abstract259)      PDF (2663KB)(67)       Save
    Objective Two in vitro drug sensitivity test methods, ager plate diffusion method (punch method) and microliquid-based dilution method, were used to observe the in vitro antibacterial activity of Medi hair spray dressing and its main components(Microbial antimicrobial peptide) against common Malassezia on the surface of seborrheic dermatitis lesions of the head. Methods Medi Hair Spray Dressing with different concentration, microbial antimicrobial peptide were used as the experimental group, and Caile lotion (concentration 2% ketoconazole lotion), blank solvent of Medi Hair Spray Dressing were used as the control group,and the diameters of the inhibition zone were compared in each group. The inhibitory effect on Malassezia in vitro was tested by agar plate diffusion method (punch method) and microdilution method, and the MIC (minimum inhibitory concentration) values were also determined. Results Medi Hair Spray Dressing and microbial antimicrobial peptide inhibited Malassezia to varying degrees in vitro,and the effect was positively correlated with drug concentration. Medi Hair Spray Dressing MIC was 0.977 μL/mL;microbial antimicrobial peptide MIC was 3.907 μL/mL. Conclusion Medi Hair Spray Dressing is safe,and has a better inhibition of Malassezia effect.
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    Clinical value of rapid on site evaluation in the diagnosis of pulmonary cryptococcosis
    WANG Hansheng, CHEN Dan, REN Tao, YOU Hui, WEI Na, LUO Guoshi, WANG Meifang
    Chinese Journal of Mycology    2023, 18 (1): 33-37,57.  
    Abstract111)      PDF (2187KB)(60)       Save
    Objective To explore the accuracy and timeliness of rapid on-site evaluation site (ROSE) in the diagnosis of pulmonary cryptococcosis by percutaneous lung biopsy. Methods A retrospective analysis of 25 cases of percutaneous lung biopsy pathologically diagnosed as pulmonary cryptococcus, including patient imaging, ROSE and pathological diagnosis results, time-consuming, and diagnosis costs was performed. Results Based on the final diagnosis, and the accuracy, sensitivity, and specificity of ROSE were 80%, 80%, and 100%, respectively. The average time for the issuance of ROSE and pathology reports were (10±1) min and (81±1.4) h, respectively; the diagnosis cost were 220 and 450 yuan, respectively. Conclusion ROSE had high accuracy and timeliness in the diagnosis of Cryptococcus neoformans pneumonia, and economic cost savings. It is worthy of application and promotion in the diagnosis of cryptococcus neoformans pneumonia in respiratory intervention.
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    Chinese Journal of Mycology    2023, 18 (3): 282-284.  
    Abstract49)      PDF (814KB)(59)       Save
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    Pulmonary infection and encephalopyosis induced by Scedosporium apiospermum:a case report and literatures review
    CAO Ya, TAN Qingche, ZHANG Jiatang, ZHAO Hong, SU Min, MA Yubao, HE Mianwang, YANG Fei, YU Shengyuan
    Chinese Journal of Mycology    2023, 18 (2): 146-150.  
    Abstract52)      PDF (2925KB)(57)       Save
    To reported one case of pulmonary infection and encephalopyosis caused by Scedosporium apiospermum, and to discuss the clinical features and the treatment. The patient’s broncholveolr lvge fluid, cerebrospinal fluid and intracranial drainage specimens were taken for fungal culture and morphological identification, and next generation sequencing. Surgical drainage and voriconazole antifungal therapy were used. According to the morphological characteristics of the strain and the results of gene sequencing, it was identified as Scedosporium apiospermum. Pulmonary and central nervous system fungal infections caused by Scedosporium apiospermum are less common and their identification relies primarily on morphological features and genetic analysis. Surgical drainage and voriconazole have a good therapeutic effect on this. However, a further follow-up is requied.
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    Pathogenic distribution andriskfactor analysis of COPD patients with secondary pulmonaryfungalinfections
    ZHANG Peiyun, DUN Jun, LUO Xinyue, HENG Xin, WANG Songping
    Chinese Journal of Mycology    2023, 18 (3): 232-235,245.  
    Abstract97)      PDF (875KB)(55)       Save
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    Pathogenic distribution characteristics of fungal bloodstream infection in Sichuan Province from 2019 to 2021
    DENG Jin, YIN Lin, JIANG Haiyan, KUANG Linghan, PENG Xi, YANG Xianggui, NI Sujiao, ZHANG Bangqin, FENG Jinfang, WANG Yanling, MA Yushan, CHEN Zongyao, ZHONG Hanyu, WU Xianli, LI Kun, WANG Ling, GAO Wei, YANG Xueqiang, ZHU Jun, CHEN Yu, ZHANG Xian, SUN Changjun, LUO Jun, LI Yumei, LI Yan, ZHANG Bin, XIE Ning, WANG Jun, XIE Yi, KANG Mei
    Chinese Journal of Mycology    2023, 18 (3): 198-204,210.  
    Abstract133)      PDF (1214KB)(55)       Save
    Objective To collect the fungal bloodstream infection data from the Sichuan Provincial Center of China Fungal Disease Surveillance System, to analyze the distribution characteristics and the susceptibility to antifungal drugs of pathogens, and to provide epidemiological data for fungal bloodstream infections in Sichuan Province. Methods The data of outpatient, emergency, and inpatient fungal bloodstream infections in 28 hospitals from January 1, 2019, to December 31, 2021, were studied. The clinical and laboratory data of fungal bloodstream infection were analyzed using Whonet 5.6 and Microsoft Excle pivot table. information. Results From January 1, 2019, to December 31, 2021, Candida accounted for 88.8% (1083/1 220) of the 1 220 fungal strains was reported by 28 hospitals. Candida albicans was the main species, accounting for 35.3% (431/1 220).and Cryptococcus neoformans being fifth in the total number of isolates, accounting for 8.3% (101/1 220). Males accounted for 56.6% (690/1 220), and female patients were less (43.4%, 530/1 220); 79.8% cases were middle-aged and elderly patients (>46 years old) and 34.8% cases were from the intensive care unit. C. albicans had the highest sensitivity to fluconazole (85.7%); resistance rates of C.tropicalis to fluconazole and voriconazole were 43.4% and 43.3%, respectively. by C. albicans isolates were dominated in Most hospitals, however, C. parapsilosis was mostly isolated in children's specialized hospitals. Conclusion The pathogens of fungal bloodstream infection and their antifungal drug susceptibility have regional characteristics. Accurately grasping the epidemiological data in the region can provide a basis for the clinical selection of antifungal drugs.
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    Chinese Journal of Mycology    2023, 18 (3): 276-281.  
    Abstract51)      PDF (895KB)(52)       Save
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    A case report: treatment of pulmonary Rhizopus infection by bronchoscopic local infusion of amphotericin B combined with argon knife
    YANG Zhipeng, LIU Chun, LIU Caihong, LIU Jianming, XIE Lihua
    Chinese Journal of Mycology    2022, 17 (4): 300-303.  
    Abstract36)      PDF (2419KB)(52)       Save
    This paper reports a case of pulmonary Rhizopus microsporus infection after haematopoietic stem cell transplantation treated by transbronchoscopic local instillation of amphotericin B combined with argon knife technique. The patient was diagnosed with hepatitis-associated severe aplastic anaemia and presented with high fever, cough, sputum and shortness of breath after peripheral blood stem cell transplantation. Imaging showed infectious lesions and partial solidity in both lungs with bilateral pleural effusion and partial swelling of both lower lungs. The pathology and second-generation gene sequencing confirmed the infection as Rhizopus microsporus, which was successfully cured by bronchoscopic treatment with a total of 16 precise instillations of amphotericin B in the right main bronchus and right lower basal segment.
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    Clinical data and drug susceptibilities of Cryptococcus neoformans infection cases in a hospital in recent 5 years in Chongqing
    ZHANG Chuanming, DAI Wei, NIU Siqiang, XU Xiuyu
    Chinese Journal of Mycology    2022, 17 (4): 269-272,293.  
    Abstract37)      PDF (834KB)(50)       Save
    Objective To investigate the infection type, treatment regimen, prognosis and drug susceptibility of Cryptococcus neoformans infection (CNI) in Chongqing area. Methods The clinical data of patients with CNI and the susceptibility of Cryptococcus neoformans isolates to 6 antifungal agents in our hospital in recent 5 years were analyzed retrospectively. Results A total of 47 cases with CNI were enrolled. Cryptococcal meningitis (72.3%) was the main type of Cryptococcus neoformans infection in our hospital, 78.7% of the patients were male, and most of the patients were non-HIV infection. In addition, 17.1% of the patients were diagnosed with cryptococcal pneumonia. Cryptococcal capsular polysaccharide antigen in serum and/or cerebrospinal fluid was assayed for 37 patients, and the positive rate was 100%. All strains were wild-type to itraconazole and voriconazole, more than 90% of strains were wild-type to 5-flucytosine and posaconazole, and 85.1% of strains were wild-type to fluconazole, but only 53.2% of strains were wild-type to amphotericin B. Good prognosis after antifungal treatment achieved in 78.0% patients. But only 16.7% of the untreated patients had a good prognosis. Conclusion Timely detection of cryptococcal capsular polysaccharide antigen can improve the diagnostic accuracy and positive rate of Cryptococcus neoformans infection. Drug susceptibility testing is also necessary after Cryptococcus neoformans was isolated. According to the Results of drug susceptibility testing, early combination therapy plays an important role in improving the prognosis of patients.
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    Chinese Journal of Mycology    2023, 18 (2): 163-166,187.  
    Abstract71)      PDF (819KB)(48)       Save
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    Chinese Journal of Mycology    2023, 18 (1): 65-70.  
    Abstract106)      PDF (871KB)(48)       Save
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