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Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Recent insights from Dectin-1-Pattern Recognition Receptor YI Xiu-li, LIU Bin, QI Xiao-long Chinese Journal of Mycology    2012, (2): 121-124.   Abstract （2249）      PDF （821KB）（975）       Knowledge map    Save The Dectin-1 is a non-Toll-like pattern recognition receptor expressed predominantly by myeloid cells, which can induce its own intracellular signalling and can mediate a variety of cellular responses, plays a key role in anti-fungal immunity. The recent studies have focused on the signalling pathways from Dectin-1, interactions between Dectin-1 and other PRRs, Dectin-1 and adaptive immunity and the role of Dectin-1 in anti-microbial immunity. Reference | Related Articles | Metrics Cited: Baidu(214) Select Glucocorticoids and invasive fungal infections:a review HAO Fei, WANG Li, ZHOU Cun-jian Chinese Journal of Mycology    2012, 7 (6): 367-371.   Abstract （1614）      PDF （796KB）（1733）       Knowledge map    Save It is well-known that hyperglucocorticoids play an important role in inducing the fungal infection,especially opportunistic fungal infection,which is attributed to the complex dysregulation of immunity caused by glucocorticoids.The patients, both those with endogenous Cushing's syndrome and, those receiving exogenous glucocorticoid therapy, are especially at risk of invasive fungal infections. A better knowledge of the interplay between glucocorticoid-induced immunosuppression and invasive fungal infections should assist in earlier recognition and establishment of specific prevention and treatment strategies in order to improve outcomes of such infections. Reference | Related Articles | Metrics Cited: Baidu(26) Select Epidemiology of tinea capitis among children in China in recent years:a retrospective analysis LI Cai-Xia, LIU Wei-Da Chinese Journal of Mycology    2011, 6 (2): 77-82.   Abstract （3197）      PDF （1458KB）（1705）       Knowledge map    Save Objective To evaluate the epidemiology of tinea capitis among children in mainland China in recent years.Methods A retrospective analysis was conducted to include literatures on tinea capitis in China published in Chinese or English language between 2000 and 2010.Results Totally 23 258 cases(male:12 745,female:10 513) summarized in 50 literatures were reported.Tinea alba was the most frequent clinical pattern(71.16% in 7 684 cases).Microsporum canis was the principle pathogen in most areas in China(67.99%),followed by Trichophyton violaceum(8.51%) and Trichophyton mentagrophytes(8.14%).While in South Xinjiang,Trichophyton violaceum was the dominant pathogen(49.19%),followed by Trichophyton schoenleinii(33.90%) and Microsporum ferrugineum(12.60%).Conclusions Microsporum canishas been remaining the dominant pathogen,but anthropophilic dermatophyte Trichophyton violaceum developed to prevalent in southeastern China.Long-term surveillance is needed for prevention and control of tinea captis. Reference | Related Articles | Metrics Cited: Baidu(19) Select Surgical management of invasive pulmonary fungal infection in patients with hematologic diseases TANG Fei-fei, XU Lan-ping, LIU Dai-hong, LIU Kai-yan, CHEN Huan, HAN Wei, ZHANG Xiao-hui, CHEN Yu-hong, WANG Feng-rong, WANG Yu, HUANG Xiao-jun Chinese Journal of Mycology    2010, 5 (2): 69-73.   Abstract （2128）      PDF （794KB）（809）       Knowledge map    Save Objective To investigate the efficacy and safety of surgical management in patients with hematology malignancies and suspected invasive pulmonary fungal infections.Methods A total of 10 patients with hematologic malignancies suspected of invasive pulmonary fungal infection and underwent pulmonary resection from April 2005 to June 2009 were recruited and analyzed.Postoperative complications and mortality were summarized to evaluate the efficiency and safty of surgery.Results All the surgery were performed under general anesthesia,including 5 thoracotomy surgery and 5 video-assisted thorascopic surgery.Three had wedge resections while seven had lobectomies.Postoperative histopathology proved invasive pulmonary fungal infection in 6 patients (60%),chronic bacterial infection in 1 patients.Postoperative complications occurred in 4 patients (40%),including pneumothorax (n=2) that absorded by itself,localized respiratory insufficiency (n=1),and hemothorax in 1 patients.Postoperative mortality within 30 d was 10%,while postoperative pulmonary IFI relapse rate was 16%.Until 31 August 2009,4 (40%) patients died with the mean follow-up period of 3.8 months (0.7-31.1 months).Conclusions Surgery is safe and helpful for diagnosis,lesion removement,prevention of IFI relapse and further immunosuppressive treatment. Reference | Related Articles | Metrics Cited: Baidu(15) Select Historical and geography studies on deep mycosis in China since 1950 to 2007 JIANG Hong-hao, ZHANG Hong, SHE Guo-rong, ZHANG Jing-wen, ZHANG Ding Chinese Journal of Mycology    2010, 5 (3): 148-153.   Abstract （1691）      PDF （838KB）（828）       Knowledge map    Save Objective To explore the historical and geography rule of deep mycosis during1950 to2007,and to provide theoretical evidence for better prevention and treatment.Methods All the literature of deep mycosis reported from 1950 to 2007 with 1980 as the intermediate year in China were reviewed.Eight geographical regions of China were set as the dividing line,while age,disease categories and related factors as the variable.All of the 15 778 cases of deep mycosis were analyzed by the SPSS 13.0.Results The incidence of deep mycosis in China increased yearly and changed gradually from centralized to decentralized in geographical distribution.The most often reported area is the Yangtze River region,followed by the Central Plains region and the Lingnan region.Highrisk population,categories,and factors related to deep mycosis in China differed in history and geography.In 1950-2007,the incidence in the young and middle-aged was high,but declined instead by those in children and the old after 1980.The categories of the disense increased significantly after 1980,of which candidasis was the most common and widespread,while rhinosporosis,copulariopsosis were still confined to restrictive local regions.Injury was the main related factor to deep mycosis before 1980,replaced by sexual contact,abuse of antibiotics,chronic diseases,and more other factors after wards.Conclusions The incidence of deep mycosis in China increases yearly in the regions with humid climate,prosperous economy and densely population,such as the Yangtze River and the Central Plains region.Combined analysis of the historical and geography characteristics is important for prevention and treatment. Reference | Related Articles | Metrics Cited: Baidu(11) Select The situation in the treatment of invasive fungal infections LI Meng, LIAO Wan-qing Chinese Journal of Mycology    2012, 7 (1): 47-51.   Abstract （2649）      PDF （801KB）（1733）       Knowledge map    Save The incidence of invasive fungal infections(IFIs) had increased over the last decade with the rise of risk population of patients who suffered from immunologic deficit.The morbidity and mortality of IFIs are high and the management of these conditions is a great challenge.This article reviews the epidemiology of the IFIs,the recently developed antifungal agents and the newly therapeutic strategies. Reference | Related Articles | Metrics Cited: Baidu(10) Select Clinical and pathological analysis on 100 cases of sporotrichosis MEI Xiang-lin, XIA Jian-xin, WANG Jing-yi, LI Xue, ZHU Wen-jing, LI Fu-qiu Chinese Journal of Mycology    2011, 6 (4): 203-206.   Abstract （1954）      PDF （1270KB）（824）       Knowledge map    Save Objective To analyze the clinical and pathological features of sporotrichosis.Method Toally 100 cases of sporotrichosis were included and clinical,pathological,and (or) spore detection rate were analysed.Results Sporotrichosis easily developed in the female which was 1.39 times of the male.The fixed form was the most common presentation with 58% incidence,while 48% mainly in 40-59 years old population.It happened most frequently in the face (49%) and upper limbs (45%).Typical pathological changes occurred in 23% cases with perineural infiltration of inflammatory cells.Spore detection rate in PAS staining was about 68%.Conclusions Fixed form is the most common presentation of sporotrichosis with variable characteristics in histopathology and 70% spore detection rate. Reference | Related Articles | Metrics Cited: Baidu(10) Select Clinical value of(1,3)-β-D-glucan in plasma on the diagnosis of deep fungal infection YANG Hui-qing, MEI Ya-ning Chinese Journal of Mycology    2011, 6 (3): 136-140.   Abstract （2113）      PDF （841KB）（968）       Knowledge map    Save Objective To study the value of plasma(1,3)-β-D-glucan and fungal culture on the diagnosis of deep fungal infection.Methods Retrospective investigation were performed on 1 868 patients with fever and treatment of immunosuppressant,corticosteroid or wide-spectrum antibiotics for a long time from August 2009 to July 2010.(1,3)-β-D-glucan concentration in plasm and culture results of peripheral blood,respiratory tract,urinary tract and intestinal canal were analysed.Results Symptoms of 757 cases in 1 868 patients were relieved after antifungal treatment.Other 1 082 cases,showed no improvement or received no antifungal drugs.Another 29 cases(eliminate) had no clinical data.In the 1 839 valuble cases,the detection ratio of(1,3)-β-D-glucan was 778/1 061 with 20 pg/mL as critical value,and 623/1 216 with 50 pg/mL as critical value respectively.Positive to negative rate in fungal culture was 457/1 382.Sensitivity(89.3%,74.9%), specificity(90.6%,94.8%),positive predictive value(PPV,86.9%,91.0%) and negative predictive value(NPV,92.4%,84.4%) obtained by different critical value showed no significal differences(P>0.05).The sensitivity,specificity,PPV and NPV of fungal culture were 49.0%,,92.1%,81.2% and 72.1% respectively.The sensitivity and specificity raised to 93.0% and 88.9% after combination of the two methods.Conclusions G-test for(1,3)-β-D-glucan detection is simple,rapid with high positive rate of fungi detection.It is suggested that G-test,fungal dtermination should be combine in the diagnosis of deep fungal infection for satisfied sensitivity and specificity. Reference | Related Articles | Metrics Cited: Baidu(10) Select Advances in the research of fungal sinusitis LIN Cheng, FAN Jing-ping, LIN Shun-zhang, LANG Jun-tian, LIU Hai-bin Chinese Journal of Mycology    2011, 6 (4): 236-240.   Abstract （2386）      PDF （894KB）（926）       Knowledge map    Save Fungal rhino-sinusitis is a common but infection in otorhinolaryngology.Patients with antibiotics,corticosteroids and immunosuppressive agents treatment,long-term radiotherapy,or with some chronic diseases such as diabetes,adustum were thought to be easilly occured with FRS.In recent years,FRS has been found in healthy people,but the reason is not clear.In this article,clinical type,diagnosis,treatment and therapeutic effectiveness of FRS are reviewed. Reference | Related Articles | Metrics Cited: Baidu(9) Select A rapid DNA extraction method for molecular identification of pathogenic fungi LU Qiao-yun, YU Jin, LIU Wei, YANG Jian-xun, MA Lei, LI Ruo-yu Chinese Journal of Mycology    2010, 5 (3): 137-140.   Abstract （1809）      PDF （948KB）（777）       Knowledge map    Save Objective To develop and evaluate a rapid DNA extraction method using Whatman FTA cards,in molecular identification of fungi.Methods DNA was extracted from 45 isolates encompassing 25 species and 6 clinical samples by whatman FTA cards.Then PCR amplification and sequencing of the internal transcribed spacer region 1 (ITS1) were conducted.Serial diluted suspension of conidia was prepared to determine the detection limit and security of this method.Results A clear DNA amplified fragment was obtained and successfully sequenced in all 45 isolates from clinical samples.The detection limit was approximately 103 cell/ mL cembined with touchdown PCR.Suspension with less than 104 cell/mL conidia could be thoroughly inactivated by FTA cards.Conclusions Whatman FTA technology thus represents an ultra-rapid method of fungal genomic DNA preparation for molecular identification from both cultures and clinical samples,and also potentially represents a powerful fungal DNA archiving and storage system. Reference | Related Articles | Metrics Cited: Baidu(9) Select Analysis of pathogenic fungi of 805 cases with onychomycosis YIN Song-chao, ZHANG Yun-qing, TAN Yong-fang, YANG Jun-ye, HUANG Huai-qiu, LI Huan, LAI Wei Chinese Journal of Mycology    2013, 8 (4): 214-216.   Abstract （1690）      PDF （776KB）（1300）       Knowledge map    Save Objective To investigate the types and composition of pathogens of onychomycosis in the Guangzhou area in recent years.Methods The mycology culture-positive patients of onychomycosis were collected and analyzed in our hospital dermatology laboratory were analyzed from June 2008 to Octobor 2012.Results In the 805 cases of mycology culture-positive pathogenic fungus,the most frequently fungus were yeast (50.55%), followed by the dermatophytes (47.95%). Mold is ranked third, accounting for (1.61%). The distibution of different fungal species have a difference at different ages.Conclusion The most frequent fungus species of onychomycosis patients in our hospital is yeast, followed by dermatophytes and molds. Age is an important factor affecting the fungal species distribution. Reference | Related Articles | Metrics Cited: Baidu(8) Select A survey of 946 cases of tinea capitis,with emphasis on possible trends in etiology and reemergence WANG Yun-ru, ZHU Min, LI Li, ZHU Jun-hao, ZHANG Chao-ying, ZHANG Qiang-qiang Chinese Journal of Mycology    2011, 6 (2): 83-86.   Abstract （1957）      PDF （686KB）（1845）       Knowledge map    Save Objective To investigate the trend and distribution of the pathogens of tinea capitis in eastern China.Methods A total of 946 patients of tinea capitis diagnosed at the Mycology laboratory,Department of Dermatology of our Hospital,from January 1993 to December 2010 were retrospectively analysed.Results During an 18-year period,a total of 946 patients with tinea capitis were diagnosed,including 410 males and 536 female,from 20 days to 93 years old.Of the 784 culture-proven tinea capitis,there were 473 cases of gray patch type tinea capitis(60.3%),216 cases of black dot type tinea capitis(27.6%),94 cases of kerion(12.0%) and only one case of tinea favosa(0.1%).The most common pathogenic fungi was Microsporum canis(470,59.9%),followed by Trichophyton violaceum(154,19.6%) and Trichophyton tonsurans(81,10.3%).Many of the patients with culture-proven Anthropophilic dermatophytes(Trichophyton violaceum in 32 cases,Trichophyton tonsurans in 12 cases and Trichophyton rubrum in 8 cases)hadhistory of intimate contact with variable kinds of animals,mostly cats and dogs.The incidence of tinea capitis reached a peak in 2001 and declined thereafter,however,10 years later,another peak occurred in 2010.Conclusions The most common type of tinea capitis in eastern China is tinea blanca and the main pathogenic fungus is Microsporum canis.Many of the tinea capitis patients with culture-proven anthropophilic dermatophyteshadhistory of intimate contact with variable kinds of animals.The latter observation may imply a reverse zoonosis phenomenon.The occurrence of a new peak of incidence suggests reemergence of the tinea capitis. Reference | Related Articles | Metrics Cited: Baidu(8) Select Combination of antifungal therapy WEI Bing, LIU Jin-yan, XIANG Ming-jie Chinese Journal of Mycology    2013, 8 (1): 61-64.   Abstract （1427）      PDF （758KB）（1432）       Knowledge map    Save Nowadays, morbidity and mortality of invasive fungal infection are increasing, especially among immunocompromised patients. However,the range of choices of existing antifungal drugs, most of which have drug toxicities and drug resistance, are limited.The combination use of antifungal drugs which has gradually applied to the clinic provides a new approach for the resistant strains infection and severe fungal infection. This review is about the effects and mechanisms of the combination therapy of different antifungal drugs. Reference | Related Articles | Metrics Cited: Baidu(7) Select Pathogenic fungi and clinical analysis of 118 cases with tinea faciei YANG Hong, GAO Zhi-qin, LI Min, DAI He-jun, YANG Lian-juan Chinese Journal of Mycology    2012, (5): 277-279.   Abstract （1640）      PDF （750KB）（795）       Knowledge map    Save Objective To assess the age distribution of the tinea faciei patients, the most common causative dermatophytes and clinical presentation of tinea faciei.Methods The patients with tinea facie treated in Dermatological Clinic during the period Jan 2012-Jun 2012 were evaluated. The diagnosis was confirmed by microscopic examination of skin specimens and culture on Sabouraud's medium with added chloramphenicol. Data of basic information, medicine histories and clinical characteristics from the 118 cases of tinea faciei were analyzed.Results Tinea faciei could occur in any age groups, however, could often been observed between 51 to 70 year old. Most frequently isolated dermatophytes are Trichophyton rubrum (68.0%), Microsporum canis (20.4%), Trichophyton mentagrophytes (9.7%), Microsporum gypseum (1.9%). Autoinoculation, contacting with infected pets and immoderate using of corticosteroid cream were the significant risk factors of tinea faciei. Using medical swab can enhance fungal smear and culture quality on the obvious inflammatory sites.Conclusion As tinea faciei was easily misdiagnosed; more attention should be given on mycological examination of skin specimens. Reference | Related Articles | Metrics Cited: Baidu(6) Select Amplified fragment length polymorphism analysis of Cryptococcus neoformans ZOU Xian-biao, WEN Hai, LIAO Wan-qing, WU Jian-hua, QIU Yun, YANG Jin-shui Chinese Journal of Mycology    2010, 5 (2): 101-104.   Abstract （2466）      PDF （885KB）（808）       Knowledge map    Save Objective To evaluate AFLP-DNA fingerprinting method in the discrimination of C.neoformans.Methods Genomic DNA from C.neoformans was digested simultaneously with two restriction enzymes.Double-stranded adapters were ligated to the ends of restricted fragments and DNA fragments were amplified by PCR using primers complementary to the adapters and the restriction site sequences.AFLP products were separated using high resolution denaturing polyacrylamide gel electrophoresis and visualized by silver staining.Results DNA fingerprint patterns of 18 C.neoformans isolates from five serotypes and clinical isolates were analyzed by AFLP.AFLP fingerprints comprised over 30 bands detected in size range 30-500 bp.Different AFLP patterns were detected in isolates of the same serotype and identical banding patterns were observed from in isolates from the same patient at different time interals and different sources from one patiant.Conclusions AFLP is highly discriminatory,and powerful epidemiological tool for improving our understanding of C.neoformans. Reference | Related Articles | Metrics Cited: Baidu(6) Select The research process of the synergistic antifungal effect of plant compositions SHEN Ling, JIANG Yuan-ying, CAO Yong-bing Chinese Journal of Mycology    2013, 8 (1): 55-60.   Abstract （1376）      PDF （780KB）（1731）       Knowledge map    Save The incidence of systemic fungal infections have increased dramatically in recent years, but now clinically available antifungal drugs are limited, and the research and development of new drugs are difficult.So combination therapy is expected to become the ideal choice for antifungal therapy. Plant compositions can play a synergistic antifungal effect with the antifungal agents in the form of a monomer or mixture.Such research is more extensive and thorough in vitro, but further study on the antifungal effects in vivo, mechanisms and clinical trials is still needed. This review focuses on the antifungal synergism of plant compositions combined with antifungal agents, so as to provide a reference for the study of the novel antifungal drugs. Reference | Related Articles | Metrics Cited: Baidu(5) Select Advances in the method of fungi diversity SHI Li-na, LIU Xiao-feng Chinese Journal of Mycology    2014, 9 (1): 60-64.   Abstract （1135）      PDF （774KB）（941）       Knowledge map    Save Fungi widely exists in nature, playing an important role in the ecosystem. The pervasive application of molecular biology technology in microbial diversity and the emergence of metagenomic breaked the limitations of traditional microbial culture method, so that improve the human's recognition of fungal diversity. This paper reviewed the main development course of studying fungal diversity approaches, introduced several commonly used methods of molecular biology on fungal diversity research, mainly including clone library PCR based, Denaturing gradient gel electrophoresis, Terminal restriction fragment length polymorphism, Real-time fluorescent quantitative PCR, Fluorescence in situ hybridization and barcoded pyrosequencing, and expounded the huge potential of metagenomics in this field. Reference | Related Articles | Metrics Cited: Baidu(5) Select The efficacy of laser therapy, drug therapy in the treatment for mild or moderate onychomycosis XU Jing, ZHUO Feng-lin, ZHANG Rui-na, ZHAO Jun-ying Chinese Journal of Mycology    2014, 9 (1): 20-23.   Abstract （1192）      PDF （786KB）（1004）       Knowledge map    Save Objective To observe the efficacy and safety of Nd:YAG 1064 nm laser therapy, itraconazole therapy in the treatment for onychomycosis.Method Sixty patients with 125 nails infected by fungi were randomly assigned to two groups. The nails in group A were treated by Nd:YAG 1064 nm laser. The nails in group B were treated by itraconazole.By following the patients of each group at 8 weeks, 16 weeks, and 24 weeks, both clinical and mycological efficacy was evaluated.Results In group A, the effective rates at 8 weeks, 16 weeks, and 24 weeks were 78.9%, 52.6% and 52.6% respectively. In group B the effective rates were 80.9%, 66.2% and 67.6% respectively. There was no significant difference in the treatment efficacy between the two groups (P<0.05).Conclusion Long pulse Nd:YAG 1064 nm laser therapy was a simple and effective method without significant complications or side effects. The difference in the treatment efficacy between the laser therapy and the drug therapy for patients with mild or moderate onychomycosis was not significant, so laser therapy could be considered in first-line therapy. Reference | Related Articles | Metrics Cited: Baidu(5) Select Multi-center epidemiological survey of pathogenic fungi of onychomycosis in China WANG Ai-ping, YU Jin, WAN Zhe, LI Fu-qiu, ZENG Jing-si, LIU Wei-da, ZHANG Qiang-qing, HAO Fei, RAN Yu-ping, XI Li-yan, LAI Wei, LI Ruo-yu Chinese Journal of Mycology    2015, 10 (4): 197-202.   Abstract （1176）      PDF （1009KB）（1444）       Knowledge map    Save Objective The aim of this study was to investigate the epidemiology of pathogenic fungi of onychomycosis in China.Methods Fungal culture and clinical analysis were performed on 1 428 cases of onychomycosis using positive direct microscopic examination.Results Eight hundred strains were isolated, the positive rate was 53.9%.The most frequently isolated fungi were dermatophytes (84%), Trichophyton rubrum accounted for 80.9%, followed by Trichophyton interdigitale and Epidermophyton floccosum. The second was yeasts (11.4%), Candida accounted for 10.1%, mainly included C.parapsilosis, C.albicans and C.tropicalis.Non-dermatophytic moulds accounted for 4.6%, included Acremonium, Aspergillus, Penicillium, Chaetomium, Fusarium and Scopulariopsis.Conclusion In China, the most frequently pathogenic fungi of onychomycosis were dermatophytes, followed by yeasts and non-dermatophytic moulds. Reference | Related Articles | Metrics Cited: Baidu(5) Select Special stains in fungal histopathology MA Tian, SONG Yue-xing, ZOU Xian-biao Chinese Journal of Mycology    2011, 6 (6): 367-369.   Abstract （2054）      PDF （342KB）（3539）       Knowledge map    Save Histological evaluation is quick and easy for pathogen identification and diagnosis in fungal infections.Some common special stains and application will be discussed in this paper. Reference | Related Articles | Metrics Cited: Baidu(5) Select Combined caspofungin and TMP-SMX treatment for PCP in AIDS patients JIANG Xue-yan, ZHANG Ren-fang, ZHENG Yu-fang, LIU Li, SHEN Yin-zhong, LU Hong-zhou Chinese Journal of Mycology    2010, 5 (3): 141-143.   Abstract （1584）      PDF （1003KB）（967）       Knowledge map    Save Objective To observe the efficacy of caspofungin combined with trimethoprim sulfamethoxazole (TMP-SMX) in the treatment of pneumocystis pneumonia (PCP) in AIDS patients.Methods Clinical feature,diagnosis and treatment procedure of nine PCP with AIDS patients were analyzed retrospectively.Results All of nine patients clinically diagnosed as PCP were administered with caspofungin and TMP-SMX.Eight patients acquired satisfied effects except one old with chronic obstructive pulmonary disease died.Conclusions Combined therapy of caspofungin and TMP-SMX has good efficacy for the treatment of PCP in AIDS patients. Reference | Related Articles | Metrics Cited: Baidu(5) Select A case of primary cutaneous mucormycosis caused by Rhizomucor variabilis ZHOU Cun-jian, WANG Li, XU Yan, ZHONG Bai-yu, HAO Fei Chinese Journal of Mycology    2011, 6 (6): 361-362.   Abstract （1833）      PDF （239KB）（1572）       Knowledge map    Save A 65-year-old man with diffused swelling plaques in the face for half a year was reported.Characteristic broad,non-septate hyphae were found in the lesion by histopathology.The isolate was identified as Rhizomucor variabilis by fungal culture,PCR and sequence analysis.The patient was diagnosied as primary cutaneous mucormycosis and improved after systemic amphotericin B for 12 days,with total dosage of 1 050 mg. Reference | Related Articles | Metrics Cited: Baidu(4) Select Experimental investigation on the detection of pathogenic molds by PCR-RFLP and multiplex PCR ZHANG Xiao-li, Lü Xue-lian, SHEN Yong-nian, Lü Gui-xia, WANG Miao-miao, GE Yi-ping, LIU Wei-da Chinese Journal of Mycology    2010, 5 (2): 105-108.   Abstract （1940）      PDF （909KB）（856）       Knowledge map    Save Objective To establish diagnositic methods for pathogenic molds using PCR-RFLP and multiplex PCR.Methods A PCR-based restriction fragment length polymorphism method was developed.ITS region of molds was amplified with universal fungal primers,and for RFLP analysis with restriction enzyme.Mutiplex PCR with 4 primer pairs was used to detect 1 template and 2 or 3 template mixtures.Sensitivity and specificity of multiplex PCR were measured.Results PCR-RFLP clearly differentiated the pathogenic molds.Multiplex PCR could amplify the corresponding 1,2 or 3 DNA fragments.Five molds were identified through distinct amplicons.Conclusions PCR-RFLP and multiplex PCR are rapid methods for the identification of pathogenic molds. Reference | Related Articles | Metrics Cited: Baidu(4) Select Inhibition of eight Chineseherbal components on M.globosa in vitro HUANG Xin, SHEN Liang-liang, XU Hong, WEN Hai Chinese Journal of Mycology    2011, 6 (2): 98-100.   Abstract （1666）      PDF （630KB）（1792）       Knowledge map    Save Objective To observe the in vitro inhibitive effect of eight kind of Chineseherbal components on ATCC standard strains of M.globosa.Methods The sensitivities of Chineseherbal components to M.globosa were determined on the basis of Clinical and Laboratory Standards Institute M27-A microdilution method.Results The MICs of Matrine,Oxymatrine,thymol and Eugenol were less than 0.98 to 1.96 μg/mL.Conclusions Matrine,Oxymatrine,thymol and Eugenolhave inhibitive effects on Malassezia in vitro and the susceptibilities could be determined by CLSI M27-A scheme. Reference | Related Articles | Metrics Cited: Baidu(4) Select Etiologic analysis of 9 566 cases of superficial mycosis in Shanghai region ZHU Jun-hao, HAN De-min, ZHAO Ying, LI Li, ZHANG Qiang-qiang Chinese Journal of Mycology    2016, 11 (3): 178-180.   Abstract （729）      PDF （803KB）（963）       Knowledge map    Save Objective To investigate the prevalence and pathogenic fungal species of superficial mycoses in Shanghai.Methods Culture and fungal identification were performed on clinical specimens collected from 9 566 patients with superficial mycoses in our hospital from Jan.2010 to Dec.2014.Results Nine thousand five hundred and sixty-six cases of superficial mycosis were included in this analysis,Onychomycosis 5 277 cases (55.16%),tinea corporis and tinea cruris 2 313 cases (24.18%),tinea manuum and tinea pedis 1 719 cases (17.97%),tinea capitis 257 cases (2.81%).T.rubrum,Candida,Rhodotorula among the 10 078 isolated pathogenic fungi strains were 50.43%,27.64% and 6.98%,respectively.Conclusions The major pathogenic fungi of superficial mycoses was Trichophyton rubrum in Shanghai area and the proportion of Candida and yeast-like fungus increased significantly,compared with the data during1994-1998. Reference | Related Articles | Metrics Cited: Baidu(4) Select Advances in the application of Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of clinically pathogenic fungi WANG Chong, ZHAN Ping, LIU Wei-da Chinese Journal of Mycology    2013, 8 (6): 369-373.   Abstract （1283）      PDF （830KB）（1184）       Knowledge map    Save Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based systems is a rapid and reliable method in identification of microorganism.With the improvement of identifiable fungal species spectrum,the application of MALDI-TOF MS systems have been introduced to identification of clinically pathogenic yeasts,yeast-like fungi,filamentous fungi and dermatophytes. Reference | Related Articles | Metrics Cited: Baidu(4) Select Clinical analysis of 56 cases of candidal balanoposthitis YANG Lian-juan, GAO Zhi-qin, GONG Wei-ming, YANG Hong, LI Ming, DAI He-jun, WEN Hai Chinese Journal of Mycology    2013, 8 (2): 87-89.   Abstract （1720）      PDF （727KB）（1237）       Knowledge map    Save Objective To investigate the clinical and mycological characteristics of candidal balanoposthitis in patients.Methods All the clinical and mycological records from 56 patients with candidal balanoposthitis at our STD clinic were reviewed.The clinical features, precipitating factors, mycological features and treatment experiences were summarized.Result The 56 patients were aged between 18 and 61 years (mean age,34.11?9.92 years), 67.9% (38/56) were aged between 20 and 40 years, 87.5% (49/56) were uncircumcised, 14.29% (8/56) were suffering from diabetes mellitus. 49 strains of Candida species were isolated from 48 patients, 44 (91.84%) were Candida albicans and 5 (8.16%) were Candida parapsilosis. All the patients were treated with sertaconazole cream topical and 5 patients with severe symptoms were combined with itraconazole oral,In the result, 76.79% (43/56) of patients showed a favorable response, followed after one month with clinical and mycological cure.Conclusion Candidal balanoposthitis is prone to the young men and Candida albicans is still the most common pathogen. Sertaconazole topical treatment is effective. Avoiding Sex is helpful to improve cure rates and re-infection. Reference | Related Articles | Metrics Cited: Baidu(4) Select Analysis of 297 cases of superficial mycosis and its pathogenic fungi strains ZHANG Wei-zheng, CHEN Li, ZHAO Jin, CHEN Jiang-han Chinese Journal of Mycology    2014, 9 (1): 28-30,35.   Abstract （1107）      PDF （1216KB）（942）       Knowledge map    Save Objective To study pathogenic fungi strains in 297 cases of superficial mycosis.Methods The clinical specimens from 297 patients with superficial mycosis, which presented positive results of the direct microscopic examination, were examined with the culture and identification of isolates of causative fungi. Sometimes accurate identification needed BioMerieux API 20C AUX or the sequences of ITS region of ribosomal DNA (rDNA) identify. The results were analysed by SPSS 17.0 statistical software.Results All 13 kinds of pathogenic fungi strains were isolated, including Trichophyton rubrum 86 strains (29.0%),Trichophyton mentagrophytes 68 strains (22.9%),Candida 59 strains (19.9%),Dematiaceous hyphomycetes 13 strains (4.4%),Aspergillus 13 strains (4.4%),Rhodotorula 12 strains (4.0%),Penicillium 9 strains (3.0%),Mucor 9 strains (3.0%),Microsporum canis 5 strains (1.7%),Cryptococcus albidus 3 strains (1.0%),Trichosporon 2 strains (0.7%),Epidermophyton floccosum 1 strains (0.3%) and 17 mixed fungal infection (5.7%).Conclusion The predominant superficial mycosis is onychomycosis, and the predominant pathogenic fungi strains is Trichophyton rubrum, but Candida infection have grown significantly. Reference | Related Articles | Metrics Cited: Baidu(4) Select The clinical efficacy of adjustment of vaginal microenvironment in the treatment of pregnancy recurrent valvovaginal candidiasis XIN Chong-min, ZHAO Tong, XU Bao-lan Chinese Journal of Mycology    2012, (5): 293-296.   Abstract （1604）      PDF （703KB）（1487）       Knowledge map    Save Objective To investigate the therapeutic effects of lactobacillus vaginal capsules for adjustment of vaginal microenvironment in the treatment of pregnancy recurrent valvovaginal candidiasis.Methods Total 266 outpatients of pregnancy recurrent valvovaginal candidiasis from December 2009 to December 2011 in our hospital were divided into experimental group and control group by random principle. The patients of experimental group took lactobacillus vaginal capsules and the antifungal treatment by clotrimazole suppository combined with traditional chinese medicine; the patients of control group took only clotrimazole suppository combined with traditional chinese medicine. The clinical effects, the rates of long-term self-healing,the long-term recurrence and the recurrence rates during delivery after the end of treatment were observed.Results The short term cure rates of the two groups were similar (P>0.05), however, there was statistic differences in the rates of long-term self-healing, the long-term recurrence rates and the recurrence rates during delivery after the application of lactobacillus vaginal capsules on experimental group (P<0.05). The rate of long-term self-healing was 28.57% in experimental group,which was significantly higher than that in control group and the long-term recurrence rate was 11.03% in experimental group, which was significantly lower than that in control group (26.92%) and the recurrence rate during delivery after treatment was 10.29% in experimental group, which was significantly lower than that in control group (29.23%).Conclusion Adjustment of vaginal microenvironment with application lactobacillus vaginal capsules and the antifungal treatment by clotrimazole suppository combined with traditional chinese medicine have good therapeutic effects on the treatment of pregnancy recurrent valvovaginal candidiasis, with high self-healing rate, low rate of recurrence and little side reaction. Reference | Related Articles | Metrics Cited: Baidu(4) Select Clinicopathologic study of 16 cases of rhino-orbitocerebral mucormycosis LI Li-li, LIU Hong-gang, PIAO Ying-shi, HE Chun-yan, ZHOU Quan, ZHANG Yang, ZHAO Zuo-tao Chinese Journal of Mycology    2010, 5 (2): 74-77,115.   Abstract （1713）      PDF （1188KB）（781）       Knowledge map    Save Objective To characterize clinicopathologic features of rhino-orbitocerebral mucormycosis (ROCM).Methods Sixteen cases of ROCM diagnosed in Beijing Tongren Hospital from 1998 to 2008 were studied retrospectively.HE,PAS and GMS stains were performed on all the paraffin-embedded tissues,one of which was also observed by transmission electron microscope.Results Fourteen patients (87.5%) had underlying conditions.The mortality was 31.3% (5 cases),including 3 patients died of ROCM and 2 died of primary diseases.Orbital (n=15) and cerebral (n=5) involvements were observed by CT and MRI.Fungal culture showed positive results in 7 cases.Pathological examination showed coagulation necrosis,fungal vasculitis and formations of granuloma in all cases,bone destructions in 9 cases and peripheral nerve fiber necrosis in 7 cases.Hyphae with high electron density and irregular shape distributed in the necrotic tissue were found under electron microscopy.Conclusions ROCM mainly occurs in immunocompromised patients,such as DM or hematological malignancies.Rhizopus and Mucor are the major pathogens.Diagnosis depends on the characteristic hyphae of Mucorales found in the lesions,combined with clinical manifestation,radiologly,laboratory and histopathologly. Reference | Related Articles | Metrics Cited: Baidu(4) Select Cryptococcal meningitis in systemic lupus erythematosus:one case report and literature review HUANG Chen, ZHU Yuan-jie, GU Ju-lin, WEN Hai Chinese Journal of Mycology    2010, 5 (4): 223-226.   Abstract （2571）      PDF （718KB）（1576）       Knowledge map    Save Objective To investigate the diagnosis and discrimination of systemic lupus erythematosus(SLE)complicated with cryptococcal meningitis.Methods One case of cryptococcal meningitis in SLE was reported and related literature was reviewed.Results The patient was treated by prednisone for a long time before central nervous system infection and misdiagnosed as a neuropsychiatric manifestation of SLE when she failed to respond to shock corticosteroid therapy.Lupus activity occurred during antifungal therapy.The symptoms were relieved after corticosteroid dosage increasing.Conclusions As the clinical findings of SLE patients with cryptococcal meningitis are non-specific and misleading,CSF India ink stain and latex agglutination test for cryptococcal antigen should be performed which effective to establish an early diagnosis.Timely diagnosis and antifungal therapy could improve the prognosis of cryptococcal meningitis in SLE patients. Reference | Related Articles | Metrics Cited: Baidu(4) Select Diagnostic value of Bronchoalveoalr Lavage in Fungal Infections JIAO Yang, BAI Chong Chinese Journal of Mycology    2010, 5 (5): 316-320.   Abstract （1487）      PDF （847KB）（1168）       Knowledge map    Save The incidence of pulmonary fungal infection increased recently.Fungal culture is gold-standard for diagnosis but restricted for time-comsuming.Bronchoscopic lavage is safe,rapid and minimally invasive to obtain bronchoalveolar lavage fluid (BALF).Cytologic analysis,antigen detection and molecula technique to BALF becoming important for rapid diagnosis of fungal infections. Reference | Related Articles | Metrics Cited: Baidu(4) Select Identification of prototheca by phenotype and molecular biology techniques ZHANG Qiang-qiang, ZHAO Ying Chinese Journal of Mycology    2010, 5 (1): 9-12,25.   Abstract （1611）      PDF （975KB）（1023）       Knowledge map    Save Objective To identify Prototheca and its varieties by phenotype and molecular biology techniques.Methods The structure and phenotypic characteristics, physiology, biochemistry and molecular biological identification, sensitivity to common anti-fungal drugs and characteristics of histopathology were reviewed,summarized and analyzed.Methods Two strains of Prototheca (P.zopfii var.hydrocarbonea and P.zopfii var .portoricensis) were identified from cerebrospinal fluid and lymph nodes by phenotypic and molecular biology techniques and relevent photographs were showed.Conclusions Diagnosis of Protothecosis mainly depends on mycological examinations including direct microscopic examination,culture,and histopathology because of no characteristic in clinical.Carbohydrates assimilation, temperature resistance and molecular biological methods would be helpful for strain identification. Reference | Related Articles | Metrics Cited: Baidu(3) Select Adjuvant management of patients with candida vulvovaginitis CHANG Lu-lu, HU Su-quan, ZHU Hong-di, WU Guo-hua Chinese Journal of Mycology    2012, 7 (6): 365-366.   Abstract （1401）      PDF （771KB）（2300）       Knowledge map    Save Objective To report 80 cases of candida vulvovaginitis received adjuvant management.Methods The adjuvant management is including methods of psychological counseling, guidance, medication and health education.Results Seventy-two cases (90.00%) recovered after a course of treatment, 6 cases (7.50%) were cured after two courses and 2 cases (2.50%) changed into the refractory candida vulvovaginitis.Conclusion Psychological counseling, guidance, medication, skin care and health education are benefit for the treatment of patients with candida vulvovaginitis. Reference | Related Articles | Metrics Cited: Baidu(3) Select Clinical investigation and pathogenic fungus analysis of 637 onychomycosis cases LIANG Xiao-bo, LI Ting-hui, HU Rong, YANG Yang, WU Jian-hua Chinese Journal of Mycology    2012, (5): 287-289.   Abstract （2102）      PDF （698KB）（830）       Knowledge map    Save Objective To analyze the clinical features, pathogenic fungus distribution and correlation between fungus and clinical patterns of onychomycosis with positive fungal culture in 637 cases.Methods Fungal culture and clinical analysis were performed on 637 cases of onychomycosis with positive result by fungal cultivation.Results DLSO were the most frequently clinical patterns. Of all the 637 cases of onychomycosis with positive fungal culture. The rates of dermatophytes, yeasts and non-dermatophytic moulds were 69.39%,18.68% and 11.93% respectively. There are differences between patients with different sex and age. Dermatophytes were the dominate strains.Conclusion In Northwest Beijing region, the dominant clinical type of onychomycosis with positive fungal culture is DLSO. The most frequent pathogenic fungus of onychomycosis is Trichophyton spp. Reference | Related Articles | Metrics Cited: Baidu(3) Select Current treatment of onychomycosis CHEN Li-hua, ZHU Hong-mei, WEN Hai Chinese Journal of Mycology    2012, 7 (3): 188-192.   Abstract （2021）      PDF （1218KB）（1091）       Knowledge map    Save Onychomycosis is the most common nail disease. The pathogens of onychomycosis include dermatophytes, yeasts of Candida species and nondermatophyte moulds. Common treatment of onychomycosis can be divided into topical and systemic therapy. Recently, new developed antifungal agents and new technologies of topical treatment will become potent complement and new direction of onychomycosis therapy. In this article, we review the current therapeutic strategies of onychomycosis in recent years. Reference | Related Articles | Metrics Cited: Baidu(3) Select The CT features in 17 cases of allergic bronchopulmonary aspergillosis ZONG Xiao-fu, WANG Hui, YU tong-fu Chinese Journal of Mycology    2013, 8 (3): 156-159.   Abstract （1535）      PDF （1172KB）（936）       Knowledge map    Save Objective To evaluate the CT features of allergic bronchopulmonary aspergillosis (ABPA), and to improve this problem.Methods The CT features in 17 cases of ABPA were analyzed retrospectively, all patients were diagnosed according to the standard diagnostic criterion of ABPA.Results The CT depicted central bronchiectasis in 17 cases.The mucoid impaction of the dilated bronchial tree were seen in 13 cases, presenting as gloved-finger and toothpaste and "Y" appearance.CT showed bronchiectasis and tree buds signs in all patients;patchy areas of consolidation or atelectasis were observed in 9 cases.Fleeting infiltration in 2 cases on following up.Conclusion s The diagnosis of ABPA should be considered in patient with long-standing asthma whose CT demonstrates central bronchiectasis with slightly hyperdense mucoid impaction. Reference | Related Articles | Metrics Cited: Baidu(3) Select Analysis of the efficacy of photodynamic therapy of condyloma acuminatum with vulvovaginal candidiasis of women infections ZHANG Yun-jie, YANG Yu-guang, GUO Xiao-juan, ZOU Xian-biao Chinese Journal of Mycology    2013, 8 (2): 101-104.   Abstract （1790）      PDF （735KB）（1113）       Knowledge map    Save Objective To evaluate the clinical effect of 5-ALA photodynamic therapy in treating patient of condyloma acuminatum (CA) with vulvovaginal candidiasis infections.Methods The 66 cases in treatment group were treated by photodynamic combined with antifungal therapy, while the 50 cases in control group by frozen combined with antifungal therapy.Results Complete response rate of treatment group was 92.4%, while that of control group was 78.0%. The difference was statistically significant (χ2=4.978, P=0.026); The cure rate of fungal infection of treatment group was 97.0%, while that of control group 86.0%. The difference was statistically significant (χ2=4.783, P=0.029). Patients were followed up for 6 months, the recurrence rate of condyloma of treatment group was 7.5%, the control group 24.0% (χ2=6.136, P=0.013). After 2 months of follow-up, the recurrence rate of fungi infection of treatment group was 4.5%, whlie the control group 16.0% (χ2=4.349, P=0.037).Conclusion 5-ALA photodynamic combined with antifungal is a safe and effective treatment of condyloma acuminatum (CA) with vulvovaginal candidiasis with low recurrence rate. Reference | Related Articles | Metrics Cited: Baidu(3) Select Recent progress of polyamines in microorganisms YAO Xiang-wen, ZHOU Mi, CAO Ying-ying, JIANG Yuan-ying Chinese Journal of Mycology    2014, 9 (2): 114-118.   Abstract （1204）      PDF （810KB）（911）       Knowledge map    Save Polyamines including putrescine, spermidine, spermine and cadaverine are polycationic molecules with hydrocarbon backbone and multiple amino groups. These subjects are essential metabolites presented in microorganisms. And polyamines involve many physiological processes of microbe, from growth to development and differentiation. Some studies found that polyamines could promote microbial cell proliferation, keep cell membrane, DNA and RNA stable, and play an important role in physiological stress, of late. This review focuses on the metabolism, transport and physiological functions of polyamines in microorganisms to provide more useful information. Reference | Related Articles | Metrics Cited: Baidu(3) Select A long-term clinical study on oral terbinafine combined with topical bifonazole cream for tinea pedis treatment ZHANG Ya-jie, LI Qing Chinese Journal of Mycology    2011, 6 (4): 220-224.   Abstract （3298）      PDF （892KB）（2992）       Knowledge map    Save Objective To find the best schedule for tinea pedis treatment.Methods A total of 250 patients with tinea pedis were divided into five groups: group A (oral terbinafine combined with 1% topical bifonazole cream for 1 week),group B (oral terbinafine for 1 week combined with 1% topical bifonazole cream for 2 weeks),group C (oral terbinafine for 2 weeks combined with 1% topical bifonazole cream for 1 week),group D (oral terbinafine combined with 1% topical bifonazole cream for 2 weeks),and group E (only 1% topical bifonazole cream for 4 weeks).Efficacy and relapse rates were analysed at the end point,week 4,24,40,56 and week 72.Results By the end of week 24,40,56 and week 72,clinical effective rate in group A,B,C and D showed statistical difference with that in group E,while group D showed statistical difference with group A and B by the end of week 40,56 and week 72.At week 24,40,56 and week 72,microbiological cure rate in group A,B,C and D showed statistical difference with that in group E,while group D showed statistical difference with group A and B at week 56 and 72.At week 40,relapse rates in group A,B,C and D were statistically different from that in group E,while at week 56 and 72,group D showed statistical difference with group A and B.Conclusions Two-week treatment with oral terbinafine and 1% topical bifonazole cream has the best therapeutic effect and lowest relapse rate. 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