Allele specific PCR for rapid detection of common gene mutations associated with the resistance of Aspergillus fumigatus

Abstract

Abstract: Objective To establish an allele specific PCR detection system for common resistance mutation genes L98H/TR34 of Aspergillus fumigatus and determine its resistance to azoles quickly and accurately. Methods Allele specific PCR method was used to simultaneously detect azole-resistant mutation genes L98H/TR34, and specificity and sensitivity of the system was evaluated. Results The real time PCR dissolution curves of all resistance gene positive Aspergillus fumigatus showed double peaks, but no mutation sites were detected in non-resistant Aspergillus fumigatus. Conclusion Allele-specific PCR can quickly detect common resistance mutant genes L98H/TR34 of Aspergillus fumigatus, with high specificity and sensitivity, simple operation and low price.

Key words: Aspergillus fumigatus, allele specific PCR, TR34/L98H

CLC Number:

R379.6

DENG Yuchen, FANG Wenjie, DENG Shuwen, CHEN Xianzhen, ZHANG Yaru, FENG Zhenyi, LING Liyan, PAN Weihua, LIAO Wanqing. Allele specific PCR for rapid detection of common gene mutations associated with the resistance of Aspergillus fumigatus[J]. Chinese Journal of Mycology, 2021, 16(6): 388-391.

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