Application of MADLI-TOF MS in rapid identification of Talaromyces marneffei

Chinese Journal of Mycology　2021, Vol. 16 　Issue (4): 229-233.

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Application of MADLI-TOF MS in rapid identification of Talaromyces marneffei

GUO Penghao¹, WU Zhongwen¹, QIU Danping², XIE Meiying³, LIU Min¹, LIAO Kang¹

1. Department of Clinical Microbiology Laboratory, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China;
2. Department of Clinical Microbiology Laboratory, Yulin First People's Hospital, Yulin 537000, China;
3. Department of Clinical Microbiology Laboratory, Fang Chenggang First People's Hospital, Fang Chenggang 538000, China

Received:2020-05-28 Published:2021-08-31

Abstract

Abstract: Objective Evaluation of the application of MADLI-TOF MS in rapid identification of T. marneffei. Methods T. marneffei isolates were collected from Guangzhou, Yulin and Fangchenggang. All the isolates identified by ITS region amplification and sequencing. The strains were pretreated by glass bead grinding and formic acid/acetonitrile extraction. The spectra of the mold and yeast phase colonies of the establishment strains on different plates and at different time points were obtained. The collected spectra were used for the establishment of the super-spectrum. The remaining isolates and other common clinical fungi isolates were used to evaluate the self-established database. Results The super-spectrum of T. marneffei with 39 characteristic peaks was established. The RUO SARAMIS database was expanded. All the validation strains were correctly identified by the expanded database, with an accuracy of 100%. Conclusion MADLI-TOF MS can quickly and accurately identify T. marneffei by the expanded database.

Key words: Talaromyces marneffei, MADLI-TOF MS, Vitek MS

CLC Number:

GUO Penghao, WU Zhongwen, QIU Danping, XIE Meiying, LIU Min, LIAO Kang. Application of MADLI-TOF MS in rapid identification of Talaromyces marneffei[J]. Chinese Journal of Mycology, 2021, 16(4): 229-233.

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Application of MADLI-TOF MS in rapid identification of Talaromyces marneffei

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