Study on the synergistic antifungal activity of glyceryl monostearate and caspofungin in vitro

Abstract

Abstract: Objective In order to improve the antifungal effect of caspofungin, we screened the synergistic effects of many compounds with caspofungin in vitro. Methods The minimum inhibitory concentration (MIC) and synergistic index (FICI) of glyceryl monostearate (GMS), caspofungin (CAS) and fluconazole (FCZ) against Candida were measured by a microdilution method. In addition, the minimum concentration of glyceryl monostearate and caspofungin used alone or in combination to inhibit the hyphae formation of Candida albicans was investigated in the RPMI 1640 and Spider medium. Results The combination of glyceryl monostearate and caspofungin showed synergistic antifungal effect on pathogenic Candida, and the synergistic index was less than 0.5. When combined with glyceryl monostearate, the concentration of caspofungin inhibiting the formation of hyphae decreased significantly. Conclusion Glyceryl monostearate could enhance the antifungal effect of caspofungin in vitro.

Key words: Candida, caspofungin, glyceryl monostearate, synergistic effect

CLC Number:

R978.5

QIU Lijuan, CHAO Wen, SHI Anzhe, YAN Lan, LYU Quanzhen, JIANG Yuanying. Study on the synergistic antifungal activity of glyceryl monostearate and caspofungin in vitro[J]. Chinese Journal of Mycology, 2021, 16(3): 145-149,165.

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