The effect of allicin on the morphological transition of Candida albicans

Abstract

Abstract: Objective To investigate the effect and mechanism of allicin on morphological transition of Candida albicans. Methods The inverted process of dynamic formation of Candida albicans hyphae was observed under microscope. CLSI-M27-A3 microdilution method was used to detect the minimum inhibitory concentration(MIC)of allicin against Candida albicans. The effect of allicin on the hyphal formation of Candida albicans in Spider liquid medium was observed under inverted microscope. qRT-PCR method was used to measure the expression of hypal related genes HWP1,ALS1,EFG1 and PDE2 of Candida albicans under the effect of allicin. Results Long hyphae began to appear in the Spider liquid medium at 6h. After 24 hours, a large number of hyphal wrapped yeast cells which interlaced tightly could be seen under inverted microscope. The MIC of allicin against to Candida albicans was 25 μg/mL. The growth of Candida albicans hyphae could be significantly inhibited by allicin in concentrations of 25-100 μg/mL in Spider liquid media. qRT-PCR results showed that the expression of hyphae related genes was down-regulated under the effect of allicin in concentrations of 25-100 μg/mL. Conclusion Allicin could significantly inhibit the morphological transition of Candida albicans, and the mechanism may correlate to the regulation of the expression level of the genes which related to hyphal formation.

Key words: Candida albicans, morphological transition, hyphal form, allicin

CLC Number:

R379.4

XIONG Yanjing, LI Rongyu, WU Yanhong. The effect of allicin on the morphological transition of Candida albicans[J]. Chinese Journal of Mycology, 2021, 16(1): 14-18.

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