Abstract: Objective To investigate the significance of detection of bronchoalveolar lavage fluid (BALF) by fungal immunofluorescent staining in the diagnosis of pulmonary fungal infection. Methods From January to December 2019, 142 BALF samples sent to our institute were tested by G/GM test and fungal immunofluorescent staining. Results In BALF of 142 cases,52 cases (36.62%) were positive by immunofluorescent staining, 49 cases were positive by G test, and 11 cases were positive by GM test. The positive rate of immunofluorescent staining was significantly higher than that of KOH microscopic examination in 27 cases (19.01%). Conclusion As a fast, convenient, efficient and direct routine detection method of fungi, direct microscopy with immunofluorescent staining was an important supplement for the detection of fungi in lavage fluid samples, and provided a fast detection method for the diagnosis of clinical pulmonary fungal infection.

Key words: lavage solution, fluorescent staining, (1,3)-β-D glucan test, galactomannan antigen test