Genotypic diversity of 11 Cryptococcus neoformans isolates from kidney transplantation patients with cryptococcosis

Abstract

Abstract: Objective To analyze genotypic diversity of 11 clinical isolates of Cryptococcus neoformans from kidney transplantation patients with cryptococcosis, which could lay the foundation for precisely therapeutic strategies on cryptococcosis associated with kidney transplantation. Methods A total of 11 C. neoformans isolates were genotyped using the Multi locus sequence typing (MLST) consensus scheme, which was recommended by the International Society for Human and Animal Mycology (ISHAM). The differences of population diversity characteristics were compared between our isolates and 22 isolates collected from 22 cryptococcosis patients with immunocompetent status. Results With the predominant ST5 (46%, 5/11), all 11 Cryptococcal isolates in this study were identified as VNⅠ molecular type (MATα type), including 5 ST types. Both results of phylogenetics and principal component analysis (PCA) showed that ST5 isolates from cryptococcosis with kidney transplant and immunocompetent status were identical. The nucleotide diversity (π) of the isolates from cryptococcosis with kidney transplant was significantly higher than that fromimmunocompetent patients. And the proportion of ST5 isolates collected from kidney transplant patients was significantly lower than that from immunocompetent patients (P=0.033). Conclusion The genetic diversity of C. neoformans isolates from cryptococcosis with kidney transplantation are significantly higher than that from cryptococcosis patients with immunocompetent status. It may be related to different STs of C. neoformans isolates with different virulence and the suppressed immunological function in patients with kidney transplantation. A more precise treatment strategies is needed in cryptococcosis patients with kidney transplantation.

Key words: Cryptococcus neoformans, genotype, phylogenetic, kidney transplant

CLC Number:

R379.5

ZHU Xin-lin, HONG Nan, ZHANG Chao, PAN Wei-hua, CAI Liang-qi, LI Xiao-jing, CHEN Min, LIAO Wan-qing. Genotypic diversity of 11 Cryptococcus neoformans isolates from kidney transplantation patients with cryptococcosis[J]. Chinese Journal of Mycology, 2020, 15(6): 338-343.

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