Study on the expression of dual specificity phosphatase in alveolar epithelial cells during Aspergillus fumigatus infection

Abstract

Abstract:

Objective To study the expression of dual specificity phosphatases (DUSPs) in alveolar epithelial cells in response to Aspergillus fumigatus infection and the release of inflammatory factors. Methods During Aspergillus fumigatus infection, ELISA method was used to detect the release of inflammatory factors in the supernatant of alveolar epithelial cells, western blot was used to detect the expression of DUSP2, DUSP6 and DUSP8 and the expression and phosphorylation level of p65, ERK1/2 and p38 in alveolar epithelial cells. Results During the infection of alveolar epithelial cells by Aspergillus fumigatus B5233 conidia, the content of TNF-α, MCP-1, IL-12p40, IL-1β, IL-6 and IL-8 in the cell supernatant gradually increased significantly. And the expression of intracellular DUSP2 gradually increased, the expression of DUSP6 gradually decreased, but the expression of DUSP8 did not change significantly. The phosphorylation level of ERK1/2 and p65 increased significantly. Conclusion In response to Aspergillus fumigatus infection, the expression of DUSP2 increased and the expression of DUSP6 was inhibited in alveolar epithelial cells, but there was no change about DUSP8 expression. At the same time, intracellular NF-κB signal and MAPK signal were activated to release a variety of inflammatory factors.

Key words: dual specificity phosphatases, Aspergillus fumigatus, alveolar epithelial cells, inflammatory factors

CLC Number:

R379.6

ZHAO Rui, CHEN Fang-yan, HAN Li. Study on the expression of dual specificity phosphatase in alveolar epithelial cells during Aspergillus fumigatus infection[J]. Chinese Journal of Mycology, 2020, 15(3): 129-133.

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