The construction of Aspergillus fumigatus mutant library and screening of itraconazole drug-resistant mutant

Abstract

Abstract:

Objective This study aim to construct a A. fumigatus mutant library and to screen the drug resistance mutant for preliminarily investigation of the mechanism of A. fumigatus drug resistance. Methods The A. fumigatus mutant library was constructed by Agrobacterium tumefaciens T-DNA insertion. For drug resistance mutant of A. fumigatus, plates that containing anti-fungal drug itraconazole were used. Results The present study obtained 1805 A. fumigatus mutants. Diagnostic PCR result showed that each tested mutant had a hygromycin marker, indicating that T-DNA were inserted into the host genome. Further, 16 strains of A. fumigatus mutants were successfully obtained by screening the mutant library using itraconazole, among which 2 exhibited obvious Amphotericin B resistance. Conclusion Sixteen A. fumigatus drug-resistant strains were obtained by construct and screening the A. fumigatus mutant library, which laid a foundation for further study on the mechanism of drug resistance of A. fumigatus.

Key words: Aspergillus fumigatus, invasive aspergillosis, T-DNA, drug resistance

CLC Number:

R379.6

LIU Jing, XU Wen, TAN Zhi-wei, LIU Yao-yao, ZHOU Ling-ling, FU Yu, LONG Nan-biao. The construction of Aspergillus fumigatus mutant library and screening of itraconazole drug-resistant mutant[J]. Chinese Journal of Mycology, 2020, 15(1): 47-51.

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