Epidemiology and antifungal susceptibilities of yeast isolates causing invasive infections

Abstract

Abstract:

Objective To investigate the species distribution and antifungal susceptibility profiles of yeast isolates causing invasive infections in Beijing Chao-Yang Hospital. Methods All the duplicated yeast isolates causing invasive infection were collected from January 2012 to December 2013 and January 2016 to December 2017. All the isolates correctly identified by MALDI-TOF MS supplemented by ITS region amplification and sequencing. Antifungal susceptibility testing to seven common agents was performed according to the CLSI broth microdilution method. Results A total of 398 yeast meet the enrollment criteria were collected and the majority was Candida spp. (n=383,96.2%). Among the Candida spp., C. albicans (n=202) was still the most common species. C. tropicalis and C. glabrata was ranked second and third position, respectively. Although the C. albicans was predominant species, all isolates were 100% susceptible to seven common antifungal agents. Six azole resistant isolates detected in this study were 3 C. tropicalis isolates, 1 C. glabrata isolate and 1 C. parapsilosis isolate. For micafungin, only 1 C. glabrata intermediate and 1 resistant isolate were detected. For amphotericin B, the MIC of all the isolates in this study were ≤ 2 μ g/mL, but for 5-flucytosine, two C. albicans isolates which MIC>64 μ g/mL were detected. Conclusion The azole resistance of common Candida spp. isolated in our hospital was lower than the level of Beijing and national surveillance data. But further detection of FKS gene mutation was needed to verify the echinocandins resistance in C. glabrata detected in this study.

Key words: antifungal susceptibility test, epidemiology, invasive fungal infection

CLC Number:

R519.3

FAN Xin, GUO Li-na, YANG Yang, ZHANG Ge, DUAN Si-meng, ZHANG Jing-jia, XU Ying-chun, YANG Chun-xia. Epidemiology and antifungal susceptibilities of yeast isolates causing invasive infections[J]. Chinese Journal of Mycology, 2020, 15(1): 10-14.

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