Abstract:

Objective To explore a simpler, less time-consuming, and more efficient glucuronoxylomannan (GXM) purification method compared with the traditional one which adapts ethanol and cetyltrimethylammonium bromide (CTAB) to specifically precipitate and purify GXM. Methods The GXM-CTAB complex was obtained by specifically precipitating 250 mL of H99 and R265 cultures with ethanol and CTAB. Then, the GXM-CTAB complex was separated using a 24:1 chloroform:isoamyl alcohol mixture. Next, GXM was precipitated by adding 3 volumes of isopropanol. Results About 7.8 mg and 18 mg of H99GXM and R265GXM were respectively obtained from 250 mL of the culture using the GXM modified purification method. The acquisition rates (GXM/GXM and GalXM mixed polysaccharides) were 39% (7.8/20) and 45% (18/40), respectively. In addition, the traditional GXM purification process takes a total of 2 weeks while the modified method only takes 8 days. Conclusion The GXM modification purification method used in this study is comparable to the extraction efficiency of the traditional method, also the purification time is shortened and the extraction process is simpler.

Key words: Cryptococcus, glucuronoxylomannan (GXM), isolation and purification, chloroform, isoamyl alcohol