Inhibitory effect of extract of Sophorae Flavescentis Radix-Cnidii Fructus Couplet medicines against Candida albicans isolate from VVC

Abstract

Abstract:

Objective To investigate the antifungal activity of extract of Sophorae Flavescentis Radix-Cnidii Fructus Couplet medicines against Candida albicans. Methods Microbroth dilution method was performed to determine the minimal inhibitory concentration (MIC). XTT reduction assay was used to evaluate the metabolic activity of C. albicans. Inverted microscopy and scanning electron microscopy (SEM) were used to observe C. albicans yeast-hypha conversion and biofilms respectively. Quantitative real time PCR (qRT-PCR) was performed to measure the expression of hypha and biofilms-related genes. Results ESCC showed significant antifungal effect and the MIC₈₀ range was 512~1024 μg/mL. The metabolic activity of C. albicans was inhibited by ESCC, and induced incomplete three-dimensional structure of C. albicans biofilms. And expression of hypha and biofilms related genes was down-regulated with ESCC exposure. Conclusion This study demonstrated that ESCC showed prominent anti-fungal activity via affecting yeast-hypha conversion and biofilms of C. albicans.

Key words: Sophorae Flavescentis Radix, Cnidii Fructus, Couplet medicines, Candida albicans, hypha, bioflims

CLC Number:

R379.4

SHI Gao-xiang, JIANG Jing-jing, WANG Yun-xia, ZHAO Ting, SHAO Jing, WANG Tian-ming, Wang Chang-zhong. Inhibitory effect of extract of Sophorae Flavescentis Radix-Cnidii Fructus Couplet medicines against Candida albicans isolate from VVC[J]. Chinese Journal of Mycology, 2019, 14(3): 147-153.

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