Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques

Abstract

Abstract:

Objective To diagnose the case of meningitis suspected of cryptococcal infection by molecular detection technique. Methods Cerebrospinal fluid samples of patients were collected, DNA was extracted, primers were designed for PCR amplification, and gene detection was performed on the amplified products by DNA chip technology. Results Sample showed positive results for Cryptococcus neoformans. Conclusion PCR amplification of primers designed from ITS conserved sequence and non-culture detection of cerebrospinal fluid samples by DNA chip technology have clinical reference value for deep fungal infections that are difficult to diagnose by traditional methods.

Key words: Cryptococcus infection, DNA chip, molecular diagnosis

CLC Number:

R519.4

WANG Guang-yu, CHEN Min, HONG Nan, WANG Sheng-qi, LIAO Wan-qing, YANG Ying. Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques[J]. Chinese Journal of Mycology, 2019, 14(2): 95-98.

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Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques

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