Pulmonary aspergilloma: a case report and laboratory study

Abstract

Abstract:

Objective Laboratory study was performed on the surgical specimens of a pulmonary fungus ball patient without fungal culture to determine the pathogen. Methods A patient with hemoptysis was found lung space occupying lesion on left upper lung and received left upper lobectomy. The postoperative histopathologic diagnosis was pulmonary fungus ball (The pathogenic fungi was the most likely Aspergillus) and the tissue was not used for fungal culture. In this study, fungal fluorescence staining, immunohistochemistry and tissue PCR assays were carried on histopathological specimens. Results Fungal fluorescence staining showed bright blue septate mycelium, with sharp-angle branch. The results of immunohistochemistry suggested that the pathogen was Aspergillus. Tissue PCR result was negative. Conclusion When lacking of fungal culture results, immunohistochemistry techniques might help determine the genus of pathogenic fungi, which was helpful for clinical diagnosis and treatment.

Key words: pulmonary fungus ball, Aspergillus, fungal fluorescence staining, immunohistochemistry, tissue PCR

CLC Number:

R519.8

SHAO Jin, YU Jin, WAN Zhe, LI Ruo-yu, WANG Ai-ping. Pulmonary aspergilloma: a case report and laboratory study[J]. Chinese Journal of Mycology, 2019, 14(1): 24-27.

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