Comparison of sensitivity of cinnamaldehyde and caspofungin to Aspergillus oryzae and their effects on cell wall of Aspergillus fumigatus

Abstract

Abstract:

Objective To study the antifungal activity of cinnamaldehyde against Aspergillus and its effect on the cell wall of Aspergillus fumigatus. To provide a basis for the development of new targeted antifungal drugs. Methods The MICs were determined by dilution on Sabouraud (4% dextrose, 1% peptone, 2% agar) dilution. Aspergillus fumigatus were prepared into 1×10⁸ CFU/mL 0.1% cinnamaldehyde and caspofungin Tween 80 (4% dextrose, 1% peptone) liquid, incubated at 37℃ for 48 h and centrifuged film, transmission electron microscopy, fluconazole as a control. Results MICs of cinnam aldehyde, caspofungin, fluconazole against Aspergillus were as followed:Aspergillus fumigatus 0.019 5 μg/mL, 0.039 1 μg/mL, >32 μg/mL; Aspergillus flavus 0.039 1 μg/mL, 0.039 1 μg/mL, >32 μg/mL. Electron microscopy showed that after 0.1% cinnamaldehyde and caspofungin acting for 48 h, the outer cell wall of Aspergillus fumigatus dissolved, the nuclei and organelles dissolved disappeared, but the cell membrane remained intact. Effect of 0.1% caspofungin on Aspergillus fumigatus cell wall and cell membrane. 0.1% fluconazole has no effect on cell wall and cell membrane of Aspergillus fumigatus. Conclusion Cinnamaldehyde and caspofungin have strong antifungal activity against Aspergillus fumigatus, but cinnamaldehyde is superior to caspofungin. Both cinnamaldehyde and caspofungin act on the cell wall of Aspergillus fumigatus without affecting the cell membrane. Fluconazole has no effect on the cell wall and cell membrane of Aspergillus fumigatus. Therefore, cinnamaldehyde will be an effective drug targeted for the treatment of Aspergillus infections.

Key words: cinnamaldehyde, caspofungin, Aspergillus fumigatus, antifungal activity, cell wall, electron microscopy

CLC Number:

DENG Jie-hua, LI JI-hong, QI Xiao-ming, WANG Gang-sheng. Comparison of sensitivity of cinnamaldehyde and caspofungin to Aspergillus oryzae and their effects on cell wall of Aspergillus fumigatus[J]. Chinese Journal of Mycology, 2018, 13(6): 345-349.

References

[1] 谢海伟,杨贤松,吕超田,等. 抗真菌药物及其作用机制研究[J].中国微生态学杂志,2015,27(12):1477-1479.
[2] WilingerB, Lackner M, Lass-Florl C, et al. Bronchoalveolar lavage ateral-flow device test for invasive pulmonary aspergillosis in solid rgan transplant patients:a semiprospective multicenter study[J]. Transplantation, 2014, 98(8):898-902.
[3] Han MS, Ahmad I. In vitro antifungal, anti-elastase and anti-keratinase activity of essential oils of cinnamomum, syzygium and cymbopogon species against aspergillus fumigatus and trichophyton rubrum[J]. Phytomedicine, 2011, 19(1):48-55.
[4] Sharma J,Gairola S,Sharma YP,et al.Ethnomedicinal Plants used to treat skindiseases by Tharu community of district Udham Singh Nagar,Uttarakhand,India[J].Ethnopharmacol,2014,158:104-206.
[5] Yang F,Ding S,Liu W,et al.Antifungal activity of 40 TCMs used individually and in combination for treatment of superficial fungal infections[J].Ethnopharmacol,2015,163:88-93.
[6] Masevhe NA,McGaw LJ,Eloff JN,The ntraditional use of plants to manage candidiasis and relatrd infections in Venda,South Africa[J].J Ethnopharmacol,2015,168:364-372.
[7] 周颖杰,李光辉.2009年美国感染病学会念珠菌病处理指南解读[J].中国循证医学杂志,2016, 16(1):20-25.
[8] 张荣发.桂皮醛的药理作用研究进展[J].中国药业,2008,17(10):75-76.
[9] 张利青,张占刚,付岩,等.桂皮醛药理作用的研究进展,中国中药杂志,2015,40(23):4568-4572.
[10] Wang GS, Deng JH, Ma YH,et al Mechanisms, Clinically Curative Effects, and Antifungal Activities of Cinnamon Oil and Pogostemon Oil Complex against three species of Candida[J]. J Tradit Chin Med,2012,32(1):19-24.
[11] 王刚生,邓洁华.桂皮油藿香油复合物对曲霉菌抗菌活性及机理研究[J].时珍国医国药,2012,23(6):1416-1417.
[12] Ruiz-Herrera J, San-Blas G. Chitin synthasis as a target for antifungal drugs[J].Curr Drug Targets-Infect Disord,2003,3(1):77-91.
[13] Naveed R, Hussain I, Tawab A, et al. Antimicrobial activity of the bioactive components of essential oils from Pakistani spices against Salmonella and other multi-drug resistant bacteria[J]. BMC Complement Altern Med, 2013,13:265.
[14] Xing F, Hua H, Selvaraj JN, et al. Growth inhibition and morphological alterations of Fusarium verticillioides by cinnamon oil and cinnamaldehyde[J]. Food Control, 2014(46):343-350.
[15] Xing F, Hua H, Selvaraj JN, et al. Growth inhibition and morphological alterations of Fusarium verticillioides by cinnamon oil and cinnamaldehyde[J]. Food Control, 2014(46):343-350.
[16] 陈辉,刘维达. 新型抗真菌药物卡泊芬净及其在临床的应用[J].国外医学皮肤病学分册,20005,31(1):9-11.
[17] 李岷,沈永年,吕桂霞,等.棘白菌素类抗真菌药[J].中国真菌学杂志,2009,4(4):249-256.
[18] 谢小梅,龙凯,方建茹,等.肉桂醛、柠檬酸抑制黄曲霉生长机制研究[J].中国公共卫生,2007,23(3):301-302.