Disruption and functional characterization of SIM1 gene in Candida albicans

Abstract

Abstract:

Objective To construct deletion mutants for Candida albicans SIM1 and explore its functions. Methods Using homologous recombination method to construct deletion mutants for Candida albicans SIM1, by measuring the growth curve, hyphae induction, adhesion epithelial cells and conducting other experiments to observe its phenotypes. Results Gene deletion mutants of SIM1 were successfully constructed. Deletion mutants of SIM1 did not significantly affect the growth and reproduction, hyphae and biofilms formation of Candida albicans, but its adhesive ability to Caco-2 cells and KB cells was obviously impaired. Furthermore deletion mutants of SIM1 were more sensitive to some drugs. Conclusions Deletion mutants of SIM1 result in changes in cell wall composition and affect the adhesion of Candida albicans to hosts.

Key words: Candida albicans, SIM1, gene knockout, cell wall, adhesion

CLC Number:

R379.4

ZHANG Xiao-long, LU Ren-yi, YAN Lan, JIANG Yuan-ying. Disruption and functional characterization of SIM1 gene in Candida albicans[J]. Chinese Journal of Mycology, 2018, 13(4): 223-228.

References

[1] NICASTRI E, PETROSILLO N, VIALE P, et al. Catheter-related bloodstream infections in HIV-infected patients[J]. Ann N Y Acad Sci, 2001, 946:274-290.
[2] NOBILE CJ, FOX EP, NETT J E, et al. A recently evolved transcriptional network controls biofilm development in Candida albicans[J]. Cell,2012, 148(1-2):126-138.
[3] LAI CC, TAN CK, HUANG YT, et al. Current challenges in the management of invasive fungal infections[J]. J J Infect Chemother, 2008, 14(2):77-85.
[4] Camougrand NM, Mouassite M, Velours GM, et al. The "SUN" family:UTH1, an ageing gene, is also involved in the regulation of mitochondria biogenesis in Saccharomyces cerevisiae[J]. Arch Biochem Biophys, 2000, 375(1):154-160.
[5] Austriaco NR Jr. Review:to bud until death:the genetics of ageing in the yeast, Saccharomyces[J]. Yeast,1996, 12(7):623-630.
[6] Skory CD, Freer SN. Cloning and characterization of a gene encoding a cell-bound, extracellular beta-glucosidase in the yeast Candida wickerhamii[J]. Appl Environ Microbiol,1995, 61(2):518-525.
[7] Hiller E, Heine S, Brunner H, et al. Candida albicans Sun41p, a putative glycosidase, is involved in morphogenesis, cell wall biogenesis, and biofilm formation[J]. Eukaryot cell, 2007, 6(11):2056-2065.
[8] Walther A, Wendland J. An improved transformation protocol for the human fungal pathogen Candida albicans[J]. Curr Genet, 2003, 42(6):339-343.
[9] Noble SM, Johnson AD. Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans[J]. Eukaryot cell, 2005, 4(2):298-309.
[10] Yan L, C te P, Li XX, et al. PalI domain proteins of Saccharomyces cerevisiae and Candida albicans[J]. Microbiol Res, 2012, 167(7):422-432.
[11] Sudbery PE. Growth of Candida albicans hyphae[J]. Nat Rev Microbiol, 2011, 9(10):737-748.
[12] Serefko AD, Poleszak EJ, Malm A. Candida albicans Denture Biofilm and its Clinical Significance[J]. Pol J Microbiol, 2012, 61(3):161-167.
[13] Pierce CG, Uppuluri P, Tristan AR, et al. A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing[J]. Nature protocols, 2008, 3(9):1494-1500.
[14] Pierce CG, Lopez-Ribot JL. Candidiasis drug discovery and development:new approaches targeting virulence for discovering and identifying new drugs[J]. Expert Opin Drug Discov 2013, 8(9):1117-1126.
[15] Fonzi WA, Irwin MY. Isogenic strain construction and gene mapping in Candida albicans[J]. Genetics, 1993, 134(3):717-728.
[16] Reuss O, Vik A, Kolter R, et al. The SAT1 flipper, an optimized tool for gene disruption in Candida albicans[J]. Gene, 2004, 341:119-127.
[17] Firon A, Aubert S, Iraqui I, et al. The SUN41 and SUN42 genes are essential for cell separation in Candida albicans[J]. Mol Microbiol, 2007, 66(5):1256-1275.
[18] Kurischko C, Weiss G, Ottey M,et al. A role for the Saccharomyces cerevisiae regulation of Ace2 and polarized morphogenesis signaling network in cell integrity[J]. Genetics, 2005, 171(2):443-455.