Welcome to Chinese Journal of Mycology,Today is Share:

Chinese Journal of Mycology 2018, Vol. 13  Issue (3): 134-138.

论文 Previous Articles     Next Articles

Evaluation of fungicidal effect of low frequency ultrasound and amphotericin B-loaded nanoparticles on C. albicans biofilm

YANG Min1, XIE Shuang1, DONG Yu1, DU Yong-hong1, LI Dai-rong2   

  1. 1. State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine, Chongqing 400016, China;
    2. First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Received:2018-01-03 Online:2018-06-28 Published:2018-06-28

Abstract:

Objective To evaluate the synergistic fungicidal effect of low frequency ultrasound combined with amphotericin B-loaded nanoparticles (AmB-NPs) on C. albicans biofilm in vitro. Method AmB-NPs were prepared by a polylactic-co-glycolic acid (PLGA) double emulsion method. XTT reduction method was performed to evaluate the effect of ultrasound with AmB-NPs on the activity of mature biofilm. The morphology of biofilm was observed by laser scanning confocal microscope. The protease and phospholipase activities of biofilm were detected by UV spectrophotometer. Results The particle size and shape of AmB-NPs were uniform. The biofilm activity of C. albicans decreased significantly after the joint action of ultrasonic irradiation and AmB-NPs compared with the control group (P<0.01). Simultaneously, the biofilm became thin and loose, with significant decreased activities of protease and phospholipase aenzyme. Conclusion Low-frequency ultrasound combined with AmB-NPs showed significant synergistic antifungal effect against C. albicans biofilm in vitro.

Key words: low frequency ultrasound, C.albicans biofilm, amphotericin B, nanoparticles, synergistic fungicidal

CLC Number: