Loss of phospholipase D in mice against Aspergillus fumigatus infection

Abstract

Abstract:

Objective To reveal the important role and the possible mechanism of the host phospholipase D in response to the infection of Aspergillus fumigatus. Methods Wild type mice and phospholipase D double genes knockout mice (pld1^-/-pld2^-/-) were infected with Aspergillus fumigatus conidia by nasal instillation. After infection, the lung tissure burden of Aspergillus fumigatus was detected by tissue homogenization and CFU counting. The secretion of inflammatory factors in BALF were detected by multi factor detection kit from eBioscience Company. Bone marrow cells of two kinds of mice were isolated and differentiated into mature macrophages (BMDM), their ability to phagocyte and kill Aspergillus fumigatus was detected by the nystatin protection method. Results Six hours post infection, the load of pulmonary fungi in pld1^-/-pld2^-/- mice was significantly higher than that of wild type group; and a large number of Aspergillus fumigatus spores were found in BALF and alveolar macrophages of pld1^-/-pld2^-/- mice. Meanwhile, the concentration of inflammatory factors in BALF of pld1^-/-pld2^-/- mice was significantly higher. In vitro experiments showed that the phagocytosis and killing ability of BMDM of pld1^-/-pld2^-/- mice was significantly reduced. Conclusion The simultaneous knockout of the pld1 and pld2 genes in mice significantly decreased the phagocytosis and fungicidal ablities of the macrophage, which might interfere mice againsting A.fumigatus infection. Meanwhile, the pld1^-/-pld2^-/- mice might secrete a large number of inflammatory factors to recruit other immune cells to kill Aspergillus fumigatus conidia.

Key words: phospholipase D, Aspergillus fumigatus infection, macrophage, inflammatory factor

CLC Number:

R379.6

LIU Xiao-yu, ZHANG Chang-jian, HU Ying-song, CHEN Fang-yan, HAN Li. Loss of phospholipase D in mice against Aspergillus fumigatus infection[J]. Chinese Journal of Mycology, 2018, 13(3): 129-133.

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