Abstract:

Objective To explore a new convenient and efficient fungi DNA extraction method,which could be used in many kinds of fungi.Methods We associated enzymic digestion with Chelex-100 to make up a new reagent for fungi DNA extraction.Results During the experiment,34 strains were tested.The DNAs of 32 strains could be obtained by our reagent,while only 27 strains' DNAs could be extracted by Takara lysis buffer.For the strains we did not successfuly obtain the DNA by the above methods,we grinded the fungi by liquid nitrogen.After that,our reagent obtained their DNA by,and only one by Takara lysis buffer.Conclusion The DNA extraction efficiency of our reagent is much better than that of Takara lysis buffer.It can be used in many kinds of fungi.For some fungi,we should firstly grind it before DNA extraction.

Key words: Protease-K, snailase, Chelex-100, DNA extraction