Transcriptome analysis of Candida biofilm formation exposed to Ultraviolet A

Abstract

Abstract:

Objective To explore the genetic expression of hyphal growth and biofilm formation of Candida albicans exposed to ultraviolet A using the transcriptome analysis.Methods In vitro Candida albicans (ATCC90028) biofilms were irradiated by a new type of LED-UVA antimicrobial device (wavelength:365 nm,intensity:13 mW/cm²,frequency:100 Hz).The differentially expressed genes between the irradiated groups and the control group were detected by RNA-Seq technology analysis and GO,KEGG enrichment analysis of genetic differences.Results A total of 1221 gene expression levels exhibited a significant difference,including 862 up-regulated genes,359 down-regulated genes.The expressions of MCR1,CBR1,MUQ1 and SHP1 were significantly increased.The expressions of RIO2 and MPP were significantly decresed.Conclusion UVA could induce apoptosis of Candia albicans by un-regulating the expression of MCR1,CBR1,MUQ1 and SHP1 and inhibit hyphal growth and development by un-regulating the expression of RIO2 and MPP.

Key words: Candida albicans, UVA, RNA-seq sequencing, differentially expressed genes

CLC Number:

R379.4

LI Jing, ZHU Dan-ting, YU Li-ying. Transcriptome analysis of Candida biofilm formation exposed to Ultraviolet A[J]. Chinese Journal of Mycology, 2017, 12(6): 332-336.

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