Comparison of azoles MICs against Candida spp.determined by the broth microdilution method at different incubation time

Abstract

Abstract:

Objective To investigate the agreement between the 24 h and 48 h azoles MICs against Candida species by broth microdilution method.Methods Antifungal susceptibility testing was carried out by broth microdilution method against 653 Candida isolates and MICs were obtained at 24 h and 48 h incubation for assessing of essential agreement (EA),categorical agreement (CA) and categorical error (Error).Results Most of Candida isolates tested could obtain sufficient growth after 24 h incubation.The overall EAs of 24 h and 48 h MICs were excellent with the three azoles for most of the species,fluconazole (91.9%),voriconazole (92.0%) and itraconazole (95.6%);the overall CAs were wonderful with fluconazole (96.9%),voriconazole (90.4%) and itraconazole (88.2%),depending on the species type tested.Very major errors (VMEs) were absent in our study,minor errors were reported most of the time.The ME/MiE for the total 653 Candida isolates were fluconazole (1.7%/2.9%),voriconazole (2.0%/10.6%) and itraconazole (1.5%/13.0%).Conclusion These data suggested that the performance 24 h MIC readings gave results similar to those of 48 h MICs and reading MICs at 24 h incubation would shorten the MIC determination of azoles and avoid errors due to trailing misinterpretation.

Key words: broth microdilution method, antifungal susceptibility, Candida, triazole, MIC

CLC Number:

R379.4

GUO Li-na, XU Zhi-peng, WANG Yao, WANG He, ZHAO Ying, XIAO Meng, XU Ying-chun. Comparison of azoles MICs against Candida spp.determined by the broth microdilution method at different incubation time[J]. Chinese Journal of Mycology, 2016, 11(6): 332-336.

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