Evaluation of the clinical application of MALDI-TOF-MS technology in detection of yeast-like fungi

Abstract

Abstract:

Objective MALDI-TOF-MS technology was evaluated the clinical application value of MALDI-TOF-MS in the rapid identification of yeast-like fungi.Methods MALDI-TOF-MS technology and traditional fungi cultural method were used to identify 142 yeast-like fungi of clinical isolates which from June 2015 to June 2016,results were recorded and analyzed.Results The rate of identification of two methods for common C.albicans,C.tropical,C.krusei and Cryptococcus neoformans were very high (100%),concordant rate was 100%;The rate of identification of two methods for uncommon C.lusitaniae,C.lipolytica and C.utilis were very low,concordant rate were 25.00%,00.00% and 00.00% respectively.Conclusion MALDI-TOF-MS had a better identification rate of yeast-like fungi,and the characteristic of simple and rapid operation provided a powerful complement for traditional manual method,which could be applied in the early rapid identification of yeast-like fungi.

Key words: MALDI-TOF-MS, yeast-like fungi, detection

CLC Number:

R446.5

ZHANG Xiao-xiao, WANG Ding-cheng, GE Wei, SHAO Hai-lian, CHENG Zhi, SU Bo, TAI Jin-ge, YANG Ming, ZHANG Qian, CHEN Jing-wen, ZHANG Hui-zhong. Evaluation of the clinical application of MALDI-TOF-MS technology in detection of yeast-like fungi[J]. Chinese Journal of Mycology, 2016, 11(5): 285-288.

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