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Chinese Journal of Mycology 2015, Vol. 10  Issue (6): 336-339.

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The evaluation of Aspergillus fumigatus infecting human bronchial epithelial cell in vitro

SHEN Liang-liang, GAO Qing-yun, HUANG Xin, ZHENG Jian-feng, HAN Yu, ZHAO Jing-jun   

  1. Department of Dermatology, Tongji Hospital, Tongji University, Shanghai 200065
  • Received:2015-07-26 Online:2015-12-28 Published:2015-12-28

Abstract:

Objective To establish a model for the relative research that simulate Aspergillus fumigatus invading human bronchial epithelial cell (HBE) and analyze the change of HBE in different time.Methods Using Aspergillus fumigatus to infect HBE in vitro and researching the change rules (biological form of HBE by adding liquid supernatant from the model,the apoptosis rate of the cells by Fluorescence Activating Cell Sorter and apoptosis gene (Bax) expression by real-time PCR).Results As the action time of Aspergillus fumigatus infecting HBE gone by,the biological form of HBE has changed,the retractile and round cells grew in number,the apoptosis rate of the cells also increased gradually and BaK expression obviously increased.Conclusion The changes of biological form,apoptosis rate and the Bax expression of HBE are evaluation system of simulating Aspergillus invading HBE in IPA which establishes in vitro and can use for relative basic and clinical research.

Key words: human bronchial epithelial cell, Aspergillus fumigatus, apoptosis, apoptosis gene

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