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Chinese Journal of Mycology 2015, Vol. 10  Issue (6): 332-335.

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Identification and knocking-out of polarized growth factor gene kipA in Aspergillus fumigates

ZHU Jun-hao1, LI Li1, ZHANG Qiang-qiang1, CHEN Yu-chong2   

  1. 1. The Lab of Medical Mycology, Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040;
    2. Dermatologic Surgery, Shanghai Dermatology Hospital, Shanghai 200050
  • Received:2015-10-19 Online:2015-12-28 Published:2015-12-28

Abstract:

Objective To construct the kipA mutant strain.Methods The vector containing upper and downstream genes of kipA linked with hygromycin resistance gene in the middle was constrctied via In-Fusion assembly.The kipA gene of Aspergillus fumigatu was replaced with hygromycin resistance gene via the method of homologous recombination.The mutant strain was identified and validated by realtime-PCR.Results The kipA deletion strains were successfully constructed.Conclusions Successful construction of kipA mutant strain would provide the foundation for the research of polarized growth and vascular invasion of Aspergillus fumigatus.

Key words: Aspergillus fumigatus, kipA gene, polarized growth

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