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Chinese Journal of Mycology 2015, Vol. 10  Issue (5): 307-311.

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MLPA application in clinical diagnosis of pathogen

FANG Wen-jie, LIU Jia, PAN Wei-hua, Liao Wanqing   

  1. Shanghai Key Laboratory of Molecular Medical Mycology, Institute of dermatology and fungal diseases, PLA Key laboratory of the fungal diseases, Department of dermatology, Changzheng hospital, Shanghai, 200433
  • Received:2014-10-26 Online:2015-10-28 Published:2015-10-28

Abstract:

As for patients diagnosed with Infectious diseases, favorable prognosis is determined by correct diagnosis and proper treatment.So it is important to diagnose clinical pathogens rapidly.Compared with other clinical test methods, MLPA is a simple, valid, and fast one which can detect several gene sequences in quantity at the same time.The steps include:the degeneration of DNA sample, hybridization, ligation, and PCR amplification.Although being at its first stage, there are already certain applications of MLPA in diagnosing and researching of clinic microorganism, and the peculiarity of easy operation, fast reaction, high pass rate and high sensitivity have reveal bright prospect of MLPA.

Key words: multiplex ligation-dependent probe amplification, pathogen, diagnosis

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