Species identification of Sporothrix clinical isolates in Northern and Southern of China

Abstract

Abstract:

Objective To identify 50Sporothrix clinical isolates in Northern and Southern of China.Methods All isolates from patients were subcultured at 25℃ and morphological characteristic was analysed by microscope and visual inspection.The total genomic DNA was extracted to amplify the partial β-tubulin gene and the internal transcribed(ITS) region by polymerase chain reaction(PCR).The phylogenetic tree was constructed by DNA sequences analyses based on datasets of ITS and a combined ITS and partial β-tubulin region using maxium likehood(ML) and neighbor-joining(NJ) methods with MEGA5 software.The Reference sequences used for phylogenetic analyses were retrieved from GenBank.Result The macroscopic morphologies and microscopic features of all isolates were comformed to Sporothrix spp.The phylogenetic tree showed that all of the isolates were clustered in a distinct clade with a type of Sporothrix globosa.Conclusion S.globosa may be the major Sporothrix existing in northern and southern of China, which can provide basis for the epidemiological investigation of Sporothrix.

Key words: Sporothrix, morphology, phylogeny, Sporothrix globosa

CLC Number:

R519.8

HE Yu, Huang Mengya, HU Qing-bi, ZHOU Xun. Species identification of Sporothrix clinical isolates in Northern and Southern of China[J]. Chinese Journal of Mycology, 2015, 10(5): 279-282.

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