The effects of T.asahii on monocyte-derived DCs phenotypic and function

Abstract

Abstract:

Objective To investigate the effects of Trichosporon asahii on the phenotypic and functional maturation of human peripheral blood monocyte-derived dendritic cells(DCs).Methods The monocyte-derived DCs of health donors were obtained by culture in vitro.In the experimental groups, DCs stimulated by different doses of heat-inactived T.asahii conidia(DCs:T.asahii=1:1, 1:5, 1:10), RPMI-1640 and lipopolysaccharide(LPS) stimulation groups were as the blank control and positive control, respectively.Shapes of the cells were observed under the inverted microscope, the rate of phagocytosis was calculated by Wright Giemsa staining.DCs maturation was analysed by using flow cytometry.Results The morphology of DCs was significantly changed during incubation.At 24 h, more than one T.asahii conidias were efficiently bound and internalized by DCs and the phagocytic rates were significantly different between the experimental groups(P<0.05).The expressions of CD80, CD86, CD83 in the experimental groups were much higher than the blank control group(P<0.05), but significantly lower than LPS group(P<0.05).Conclusion T.asahii can change the morphology of DCs and promote the expression of surface molecules, which enhance the maturation of the dendritic cells.

Key words: T.asahii, dendritic cells, surface molecules

CLC Number:

R379.9

Wang Ruili, AO Jun-hong, LIAO Yong, YANG Rong-ya, YANG Dong-qian, LV Xue-lian, Chen Shanshan. The effects of T.asahii on monocyte-derived DCs phenotypic and function[J]. Chinese Journal of Mycology, 2015, 10(5): 266-271.

References

[1] Girmenia C,Pagano L,Martino B,et al.Invasive infections caused by Trichosporon species and Geotrichum capitatum in patients with hematological malignancies:a retrospective multicenter study from Italy and review of the literature[J].Clin Microbiol,2005,43(4):1818-1828.
[2] 杨蓉娅,敖俊红,王文岭,等.阿萨希丝孢酵母引起播散性毛孢子菌病国内首例报告[J]中华皮肤科杂志,2001,34(5):329-332.
[3] Pfaller MA,Diekema DJ.Rare and emerging opportunistic fungal pathogens:concern for resistance beyond Candida albicans and Aspergillus fumigatus.
[J].Clin Microbiol,2004,42(10):4419-4431.
[4] Romagnoli G,Nisini R,Chiani P,et al,The interaction of human dendritic cells with yeast and germ-tube forms of Candida albicans leads to efficient fungal processing,dendritic cell maturation,and acquisition of a Th1 response-promoting function[J].Leukoc Biol,2004,75(1):117-126.
[5] Santiago K,Bomfim GF,Criado PR,et al.Monocyte-derived dendritic cells from patients with dermatophytosis restrict the growth of Trichophyton rubrum and induce CD4-T cell activation[J].PLoS One,2014,9(11):e110879.
[6] Gafa V,Remoli ME,Giacomini E,et al.In vitro infection of human dendritic cells by Aspergillus fumigatus conidia triggers the secretion of chemokines for neutrophil and Th1 lymphocyte recruitment[J].Microbes Infect,2007,9(8):971-980.
[7] Wozniak KL,Levitz SM.Cryptococcus neoformans enters the endolysosomal pathway of dendritic cells and is killed by lysosomal components.
[J].Infect Immun,2008,76(10):4764-4771.
[8] Kelly RM,Chen J,Yauch LE,et al.Opsonic requirements for dendritic cell-mediated responses to Cryptococcus neoformans[J].Infect Immun,2005,73(1):592-598.
[9] Syme RM,Spurrell JC,Amankwah EK,et al.Primary dendritic cells phagocytose Cryptococcus neoformans via mannose receptors and Fcγ receptor Ⅱ for presentation to T lymphocytes[J].Infect Immun,2002,70(11):5972-5981.
[10] Li DY,Gu C,Min J,et al,Maturation induction of human peripheral blood mononuclear cell-derived dendritic cells[J].Exp Ther Med,2012,4(1):131-134.
[11] 丛林,夏志宽,杨蓉娅.补体对RAW264.7小鼠巨噬细胞吞噬阿萨希毛孢子菌的增强作用[J].实用皮肤病学杂志,2013,6(4):195-197.
[12] Ohnmacht C,Pullner A,King SB,et al.Constitutive ablation of dendritic cells breaks self-tolerance of CD4 T cells and results in spontaneous fatal autoimmunity[J].Exp Med,2009,206(3):549-559.
[13] Verdan FF,Faleiros JC,Ferreira LS,et al.Dendritic cell are able to differentially recognize Sporothrix schenckii antigens and Promote Th1/Th17 response in vitro[J].Immunobiology,2012,217:788-794.
[14] Loures FV,Pina A,Felonato M,et al.MyD88 signaling is required for efficient innate and adaptive immune responses to Paracoccidioides brasiliensis infection[J].Infect Immun,2011,79(6):2470-2480.
[15] Kim SK,Son CG,Yun CH,et al.Hericium erinaceum induces maturation of dendritic cells derived from human peripheral blood monocytes[J].Phytother Res,2010,24(1):14-19.
[16] Buentke E,Heffler LC,Wallin RP,et al.The allergenic yeast Malassezia furfur induces maturation of human dendritic cells[J].Clin Exp Allergy,2001,31(10):1583-1593.
[17] Vittore Cereda,a Matteo Vergati,a Ngar-Yee Huen,et al.Maturation of human dendritic cells with Saccharomyces cerevisiae(yeast) reduces the number and function of regulatory T cells and enhances the ratio of antigen-specific effectors to regulatory T cells[J].Vaccine,2011,29(31):4992-4999.
[18] 曹雪涛.免疫学前沿进展[M].北京:人民卫生出版社,2009:68-69.
[19] Mellman I.Dendritic cells:master regulators of the immune response[J].Cancer Immunol Res,2013,1(3):145-149.
[20] Newman SL,Holly A.Candida albicans is phagocytosed,killed,and processed for antigen presentation by human dendritic cells[J].Infect Immun,2001,69(11):6813-6822.
[21] Bozza S,Gaziano R,Spreca A,et al.Dendritic cells transport conidia and hyphae of Aspergillus fumigatus from the airways to the draining lymph nodes and initiate disparate Th responses to the fungus[J].Immunol,2002,168(3):1362-1371.
[22] Susanne Herbst,Anand Shah,Maria Mazon Moya.et al.Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus[J].EMBO Mol Med,2015,7(3):240-258.
[23] Wang Runchao,Wan Zhe and Li Ruoyu.Th and Treg response induced byAspergillus fumigatus pulsed dendritic cells in vitro[J].Chin Med J,2014,127(20):3616-3622.
[24] Huston SM1,Li SS,Stack D,et al.Cryptococcus gattii is killed by dendritic cells,but evades adaptive immunity by failing to induce dendritic cell maturation[J].Immunol,2013,191(1):249-261.
[25] Retini C,Vecchiarelli A,Monari C,et al.Encapsulation of Cryptococcus neoformans with glucuronoxylomannan inhibits the antigen-presenting capacity of monocytes[J].Infect Immun,1998,66(2):664-669.