Comparison of DNA extraction methods from Sporothrix globosa

Abstract

Abstract:

Objective To find a rapid, effective and easy-to-perform DNA extraction protocol from Sporothrix globosa.Methods Four methods, such as CTAB-extraction, guanidine thiocyanate boiling-extraction, alkaline lysis-extraction, DNA extraction kit, were compared based on Sporothrix globosa isolates from different regions.The purity and yield of the DNA were determined by ultraviolet absorption spectrum.The DNA quality was evaluated with agarose gel electrophoresis and PCR amplification.Results It showed that all methods could get DNA, but alkaline lysis-extraction was better than others for the purity and yield of the DNA.Based on degenerated primers of fragments of the calmodulin genes for PCR, we found all samples showed bright stripe.Conclusion Alkaline lysis-extraction is the most suitable method for extracting high quality DNA from Sporothrix globosa.

Key words: Sporothrix globosa, Alkaline lysis-extraction, CTAB-extraction, Guanidine thiocyanate boiling-extraction, DNA extration

CLC Number:

R379.9

SHI Ying, CUI Yan, ZHAO Li-pei, SONG Yang, LI Shan-shan. Comparison of DNA extraction methods from Sporothrix globosa[J]. Chinese Journal of Mycology, 2015, 10(4): 216-219.

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