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Chinese Journal of Mycology 2015, Vol. 10  Issue (4): 207-209.

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In vitro antifungal activity of tacrolimus combined with antifungals against Candida spp. and dermatophytes

CHEN Chun1, CHEN Qi-hong1, SUN Yi1, GAO Lu-juan2, ZENG Tong-xiang1   

  1. 1. Department of Dermatology and Venereology, Jingzhou Central Hospital, Jingzhou 434100, China;
    2. Department of Dermatology, Zhongshan Hospital Fudan University, Shanghai, 200032, China
  • Received:2015-04-11 Online:2015-08-28 Published:2015-08-28

Abstract:

Objectives To investigate the in vitro antifungal susceptibility of FK-506 combined with antifungals to Candida spp.and dermatophytes. Methods The minimal inhibitory concentrations (MICs) of Candida spp.against ketoconazole and dermatophytes against terbinafine were assayed using CLSI M27-A3 and M38-A2 microdilution method,respectively.By using broth microdilution checkerboard technique system,we evaluated the combined antifungal activity of tacrolimus with ketoconazole or terbinafine against Candida spp.and dermatophytes,respectively. Results The MIC50 and MIC90 of ketoconazole against Candida spp.were 4 μg/mL and 8 μg/mL,respectively.The MIC50 and MIC90 of terbinafine against dermatophytes were 0.06 μg/mL and 0.125 μg/mL,respectively.The result showed that the combination of tacrolimus and Ketoconazole or Terbinafine had synergistic inhibitory activity against Candida spp.(53.5%,n=15) and dermatophytes (46.7%,n=15). Conclusion The MICs of antifungals can be reduced partly when combined with low concentration of FK-506 against Candida spp.and dermatophytes,and no antagonism was found.

Key words: Candida spp., Tacrolimus (FK-506), dermatophytes, antifungl, in vitro susceptibility

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