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Chinese Journal of Mycology 2015, Vol. 10  Issue (3): 163-167.

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An investigation on RNA-interference method applicable for the filamentous fungi

ZHANG Zhen-ying1, HOU Bin-bin2, LIU Xia2   

  1. 1. Department of Dermatology, HongKong University-Shenzhen Hospital, Shenzhen 518053;
    2. Department of Dermatology, 2nd Affiliated Hospital of Dalian Medical University, Dalian 116023
  • Received:2014-12-29 Online:2015-06-28 Published:2015-06-28

Abstract:

Objective To obtain a RNA-interference method mediated by Agrobacterium tumefaciens applicable for gene research on filamentous fungi.Methods Gene recombination and interference system selection were used.Results The recombinant vector PCB309-pfgrt was obtained and used in interfering the expression level of two-component DRK1 gene successfully.Conclusions This method optimized the transformation system,solving the problem of high efficient RNA interference vector construction,which has wide application prospect in genetic research in filamentous fungi.

Key words: recombinant vector, RNA-interference, filamentous fungi, Agrobacterium tumefaciens

CLC Number: