Effect of targeted gold nanoparticles on viability of Cryptococcus neoformans in vitro research

Abstract

Abstract:

Objective To design targeted gold nanoparticles (GNP) conjugated with anti-Cryptococcus neoformans antibody,to explore targeted gold nanoparticles-mediated photothermal therapy of Cryptococcus neoformans in vitro.Methods Gold nanoparticles were synthesized according to seed-mediated template-assisted protocol,conjugated with anti-Cryptococcus neoformans antibody(Ab).Cryptococcus neoformans were incubated with targeted gold nanoparticles,then exposed to near-infrared (NIR) light.Cell viability was evaluated.Results Targeted gold nanoparticles were synthesized successfully and can be used to selectively target Cryptococcus neoformans cells and following NIR laser exposure,can be used to significantly reduce cell viability.Conclusions These results demongstrated that Ab-mediated immunity gold nanoparticles can enhance NIR photothermal treatments effect on Cryptococcus neoformans.

Key words: gold nanoparticles, Cryptococcus neoformans, photothermal treatments

CLC Number:

R379.5

XIAO Qin, CHEN Min, XU Nan. Effect of targeted gold nanoparticles on viability of Cryptococcus neoformans in vitro research[J]. Chinese Journal of Mycology, 2015, 10(3): 134-138.

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