Morphological observation and genetic identification of a variant of granular type Trichophyton rubrum isolated from tinea corporis and cruris

Abstract

Abstract: Objectives To investigate the morphological features and sequences of ribosomal genes in a variant of granular type Trichophyton rubrum isolated from tinea corporis and cruris.Methods The morphological features of one T.rubrum strain recovered from a patient with tinea corporis and cruris were observed. The ribosomal internal transcribed spacer (ITS), D1/D2 and non-transcribed region were determined by PCR.Results The phenotype of the isolate produced antlers mycelium, macroconidium had hollow phenomenon, unilateral budding and it grew at 42℃. The sequences of ITS and D1/D2 were identical to T.rubrum in the GenBank, but sequences of TRS-1 and TRS-2 in the non-transcribed region were different from the reference strains by 2 and 1 base, respectively.Conclusion The strain might be a variant of granular type T.rubrum.

Key words: Trichophyton rubrum, tinea corporis, genetic identification

CLC Number:

R379.2

YANG yan-ping, LI wen, OU ru-jun, CHEN he-lian, HUANG wen-ming. Morphological observation and genetic identification of a variant of granular type Trichophyton rubrum isolated from tinea corporis and cruris[J]. Chinese Journal of Mycology, 2014, 9(5): 279-282,278.

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