Using LC-MS/MS for determination of Gliotoxin in vitro model of human bronchial epithelial infected by Aspergillus fumigatus

Abstract

Abstract: Objectives To establish a LC-MS/MS method for the in vitro determination of Gliotoxin (GT) in co-culture model of human bronchial epithelial (HBE) infected by Aspergillus fumigatus (AF).Methods Developing co-cultured model of HBE infected by AF,the concentration of GT were detected by LC-MS/MS in different periods.Results The GT concentration of supernatant from co-culture increased over time (P<0.05) and the recovery of GT was 68.7%-72.6%.Conclusion This method is sensitive, simple and accurate, and suitable for the determination of GT in the co-culture model.

Key words: human bronchial epithelial cell, Aspergillus fumigatus, glioxin, LC-MS/MS, determination

CLC Number:

R379.6

SHEN Liang-liang, Han yu, Zheng Jian-feng, Zhao Jing-jun. Using LC-MS/MS for determination of Gliotoxin in vitro model of human bronchial epithelial infected by Aspergillus fumigatus[J]. Chinese Journal of Mycology, 2014, 9(5): 268-271.

[1]	SHEN Liang-liang, GAO Qing-yun, HUANG Xin, ZHENG Jian-feng, HAN Yu, ZHAO Jing-jun. The evaluation of Aspergillus fumigatus infecting human bronchial epithelial cell in vitro [J]. Chinese Journal of Mycology, 2015, 10(6): 336-339.
[2]	JIA Zhen-yu, LI De-dong, JIANG Yuan-ying, WANG Yan. Key virulence factors of Aspergillus fumigatus：an update [J]. Chinese Journal of Mycology, 2013, 8(4): 241-245.
[3]	HAN Gai-ge, JIA Xiao-dong, HAN Xue-lin, ZHAO shuai, HAN li. The application of long-fragment fusion PCR in the construction of rho1-compelement strain of Aspergillus fumigatus [J]. Chinese Journal of Mycology, 2013, 8(3): 129-133.
[4]	ZHANG Jin-qing, LIU Wei, TAN Jing-wen, WAN Zhe, LI Ruo-yu. Analysis of the growth characteristics of Aspergillus fumigatus in different temperature and nutrient media [J]. Chinese Journal of Mycology, 2013, 8(1): 6-9.
[5]	MA Dong-mei, JI Ya-juan, YANG Fang, LIU Wei, WAN Zhe, LI Ruo-yu. The effect of extra copies of sho 1 or pbs 2 gene on the adaptive ability of Aspergillus fumigatus to several stresses [J]. Chinese Journal of Mycology, 2012, (4): 193-198.
[6]	GAO Lu-juan, YU Jin, LI Ruo-yu. Re-identification,standard CSP typing and in vitro susceptibility of Aspergillus fumigatus complex [J]. Chinese Journal of Mycology, 2011, 6(6): 328-331,336.
[7]	WU Ran, CHENG Bo, JIA Min. Therapeutic efficacy of itraconazole against Aspergillus fumigatuskeratitis [J]. Chinese Journal of Mycology, 2011, 6(6): 332-336.
[8]	YANG Fang, LIU Wei, WAN Zhe, JI Ya-juan, LI Ruo-yu. The role ofpbs2 gene in the heat stress and cell wall stress of Aspergillus fumigatus [J]. Chinese Journal of Mycology, 2011, 6(4): 199-202.
[9]	WU Ran, CHENG Bo, JIA Min. Experimental study on corneal aspergillosis [J]. Chinese Journal of Mycology, 2011, 6(3): 141-144.
[10]	ZHAO Zuo-tao, SUN Zheng, WAN Zhe, ZHU Ping, LI Ruo-yu. Prediction and identification of new HLA-A-* 0201-restricted CD8-+ CTL epitopes from Aspergillusfumigatus antigen Asp f16 [J]. Chinese Journal of Mycology, 2011, 6(2): 87-92.
[11]	ZHAO Zuo-tao, SUN Zheng, WAN Zhe, WANG Xiao-hong, LI Ruo-yu. Generation and expansion of Aspergillus fumigatus specific T cells in vitro [J]. Chinese Journal of Mycology, 2010, 5(5): 257-263.

Using LC-MS/MS for determination of Gliotoxin in vitro model of human bronchial epithelial infected by Aspergillus fumigatus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments