Abstract: Objective To investigate the in vitro susceptibility testing of terbinafine against 24 strains of Black yeast like fungi.Methods CLSI M38-A2 guideline was applied. The media containing the fungi in the concentration of 0.5×10⁴-4×10⁴ CFU/mL were incubated at 30 for 5-7 days. The MIC and MEC values were observed.Results The tested 24 strain of black yeast like fungi were susceptible to terbinafine, MEC range:0.125 μg/mL-4 μg/mL,MEC₉₀:2 μg/mL,MEC₅₀:0.25 μg/mL,GM:0.392 9 μg/mL,Five strains had 100% growth inhibition,MIC range:1-4 μg/mL,MIC₅₀:2 μg/mL;MIC₉₀:4 μg/mL.Conclusion Terbinafine is active aganist black yeast like fungi in order Chaetothyriales. Most strains had MEC which 50% growth inhibition by terbinafine.

Key words: black yeast, antifungal susceptibility tests, terbinafine