Abstract: Objective To isolate and identify the phenotype of pathogenic fungus,to get molecular diagnosis and in vitro drug sensitivity of Fusarium in a patient with fungemia after multiple organs injury.Methods A young patient diagnosed as multiple organs injury, and his blood was collected from deep veins and cultured for both bacterium and fungi. The isolated fungus from the blood culture were identified further with phenotype and molecular methods. Finally, E-test was used to detect the antifungal susceptibility.Results The blood culture was negative for bacteria, but positive for fungi. The isolate was confirmed as "Fusarium solani" according to the morphology of the fungus and the Results of phenotypic and molecular identification. The isolate was sensitive to AMB and VOZ, but resistant to ITZ, CS and FCZ.Conclusion Fusarium could cause local and disseminated infections in humans, and it was always vital and negative to most of antifungal drugs. It is necessary to perform blood fungal culture for the patients who were still febrile even after the debridement and antibiotic therapy. The fungal strain could be identified by the phenotype and molecular analysis.

Key words: Fusarium, blood culture, fungemia, phenotype, molecular technology