Abstract: Objective To dissect genomic changes in gene expression of mouse brain microvascular endothelial cells infected by Cryptococcus neoformans for providing new clues of fungal predilection for the central nervous system.Methods By comparing the gene expression profiles of bEnd.3 cells before and after infected by Cryptococcus neoformans of different serotypes, the changes of some important genes were found and then validated by RT-PCR.Results After comparison, we got 383 differentially expressed genes ,including 263 down-regulated and 120 up-regulated ones. And then we selected adhesion molecule gene CDH10 and selenium binding protein 1 gene SELENBP1 and validated their expression levels by RT-PCR . The results were in line with the microarray data.Conclusion Cryptococcus neoformans caused down-regulation of adhesion molecule CDH10 and up-regulation of SELENBP1 in brain microvascular endothelial cell might contribute to the yeast's invasion to host blood-brain barrier (BBB).

Key words: brain microvascular endothelial cell, gene chip, Cryptococcus neoformans