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Chinese Journal of Mycology　2014, Vol. 9 　Issue (3): 139-142,146.

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The influence of culture media with different nitrogen and carbon nutrient sources on the biological characters of Trichophyton rubrum

HU Chan1,2, ZHU Hong-Mei2, TAN Hong-Yue1, LI Ping2, SONG Wei-fang3, GU Jun1, WEN Hai2   

  1. 1. Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
    2. Shanghai Key Laboratory of Molecular Medical Mycology and PLA Key Laboratory of Fungal Diseases, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
    3. Department of Dermatology, Affiliated Chenggong Hospital of Xiamen University Xiamen No.174 Hospital, Xiamen 361003, China
  • Received:2013-12-23 Online:2014-06-28 Published:2014-06-28

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Abstract:

Objective To find the influence of culture medium with different kinds of nitrogen and carbon source on the growth morphology of Trichophyton rubrum.Method Point inoculated the Trichophyton rubrum on potato dextrose medium,trypsin agar medium, keratin glucose medium and glucose agar medium. Observed the morphology and tested the area of the colony after 2 weeks of incubation. Compared the growth morphology of Trichophyton rubrum when inoculated with and without glucose. Compared the influence of different keratin concentration to the growth morphology of Trichophyton rubrum.Result Different protein as nithogen source in different medium could all promote the growth of Trichophyton rubrum.Yet Trichophyton rubrum growed better in keratin culture and produced more red pigment. Glucose added to the keratin culture as carbon source could effectively promote the growth of Trichophyton rubrum and the production of red pigment.Conclusion The concentration of keratin in the culture medium can affect the growth of Trichophyton rubrum,and the glucose is the key factor to the production of red-pigment for Trichophyton rubrum. The overall understanding of metabolic mechanism of Trichophyton rubrum might provide a good basis for further study and even a good strategy of the antifungal research.

Key words: Trichophyton rubrum, keratin, glucose, red pigment

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