Synergistic effect of baicalein in combination with fluconazole on Candida albicans biofilm

Abstract

Abstract:

Objective To investigate the effect of baicalein (BE) in combination with fluconazole (FLC) on Candida albicans biofilm formation.Methods The inhibition of C.albicans biofilm by BE in combination with FLC was determined by confocal scanning laser microscopy and XTT methods. The water-hydrocarbon two-phase assay was used to measure the cell surface hydrophobicity (CSH). The expression of CSH1, EFG1, HWP1, ALS1 mRNA was measured by Real time RT-PCR.Results BE in combination with FLC could inhibit the formation of C.albicans biofilm. The cell surface hydrophobicity in the BE and FLC-treated group was lower than that in the groups treated by BE or FLC alone. Consistent with this, BE and FLC-treated biofilm cells expressed lower levels of CSH1, EFG1, HWP1 mRNA than the cells grown in the presence of BE or FLC alone.Conclusions BE in combination with FLC could inhibit the formation of C.albicans biofilm.

Key words: Candida albicans, baicalein, fluconazole, biofilm

CLC Number:

R379.4

ZHAO Liu-ya, JIANG Jing-chen, YAO Xiang-wen, CAO Ying-ying, JIANG Yuan-ying. Synergistic effect of baicalein in combination with fluconazole on Candida albicans biofilm[J]. Chinese Journal of Mycology, 2014, 9(2): 70-74.

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