The study of a simple in vitro onychomycosis model

Abstract

Abstract: Objective To determine a simple, novel and economic in vitro onychomycosis model.Method An innovatively designed "O" ring was applied to localize the fugal suspension on the ventral surface of the porcine hoof, then 50 μL of fungal suspension was applied. The hoof membranes were cultured in sterile petri dishes. The dynamic penetration of hoof plates was observed with gross appreance and histopathological examination.Result In this study we developed a novel in vitro model of onychomycosis with porcine hoof in which we observed the Trichophyton rubrum,Trichophyton mentagrophytes and Candida albicans dynamic permeation to the dorsal of porcine hoof both by gross appreance and histopathological examination and this model also enables one to evaluate how the fungi behave in the nail plate and changes in the localization.Conclusion The model facilitates monitoring of the nail pathogenicity.

Key words: onychomycosis, in vitro model, dermatophytes, Candida albicans

CLC Number:

R756.4

HU Chan, ZHU Hong-mei, TAN Hong-yue, LI Ping, ZHAO Jin, CHEN Li, WEN Hai, SONG Wei-Fang. The study of a simple in vitro onychomycosis model[J]. Chinese Journal of Mycology, 2013, 8(5): 265-268.

References

[1] Heikkila H, Stubb S. The prevalence of onychomycosis in Finland[J]. Br J Dermatol,1995, 133(5): 699-703.
[2] Effendy I, Lecha M, Feuilhade de Chauvin M, et al. Epidemiology and clinical classification of onychomycosis[J]. J Eur Acad Dermatol Venereol,2005,19(Suppl 1): 8-12.
[3] Hay R. Literature review. Onychomycosis[J]. J Eur Acad Dermatol Venereol,2005,19 (Suppl )1: 1-7.
[4] Choi B, Tsu L, Chen E,et al. Determination of chemical agent optical clearing potential using in vitro human skin[J]. Lasers Surg Med,2005,36(2):72-75.
[5] Ghannoum MA, Hajjeh RA, Scher R, et al. A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns[J]. J Am Acad Dermatol, 2000, 43(4): 641-648.
[6] Lateur N, Mortaki A, Andre J. Two hundred ninety-six cases of onychomycosis in children and teenagers: a 10-year laboratory survey[J]. Pediatr Dermatol, 2003, 20(5): 385-388.
[7] Elewski BE, Charif MA. Prevalence of onychomycosis in patients attending a dermatology clinic in northeastern Ohio for other conditions[J]. Arch Dermatol,1997,133(9): 1172-1173.
[8] Hay R. Literature review. Onychomycosis[J]. J Eur Acad Dermatol Venereol,2005,19 (Suppl )1: 1-7.
[9] Elewski BE, Charif MA. Prevalence of onychomycosis in patients attending a dermatology clinic in northeastern Ohio for other conditions[J]. Arch Dermatol,1997,133(9): 1172-1173.
[10] Jesudanam TM, Rao GR, Lakshmi DJ, Kumari GR. Onychomycosis: a significant medical problem[J]. Indian J Dermatol Venereol Leprol, 2002,68(6): 326-329.
[11] Veer P, Patwardhan NS, Damle AS. Study of onychomycosis: prevailing fungi and pattern of infection[J]. Indian J Med Microbiol,2007,25(1): 53-56.
[12] Williams HC. The epidemiology of onychomycosis in Britain[J]. Br J Dermatol,1993,129(2): 101-109.
[13] Ellis DH, Watson AB, Marley JE, Williams TG. Non-dermatophytes in onychomycosis of the toenails[J]. Br J Dermatol,1997,136(4): 490-493.
[14] Kretschmar M, Felk A, Staib P, et al. Individual acid aspartic proteinases (Saps) 1-6 of Candida albicans are not essential for invasion and colonization of the gastrointestinal tract in mice[J]. Microb Pathog,2002,32(2): 61-70.

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