Studies on the relationship between hemolytic activity and biofilm formation ability of Trichosporon asahii isolates and their genotypes

Abstract

Abstract: Objective To investigate the in vitro hemolytic ability and biofilm formation capacity of Trichosporon asahii strains isolated from urinary tract infected patients and their relationship with corresponding genotypes. To provide laboratory basis for clinical diagnosis and treatment of Trichosporon asahii.Methods Glass beads method and PCR technology were used to extract total DNA and determine the specific genotypes of clinical isolates (primers for IGS1 area), respectively; Plate method and XTT reduction assay were carried out to detect the hemolytic activity and biofilm formation capacity of clinical isolates.Results Ten Trichosporon asahii strains isolated from urinary tract infections belonged to genotype Ⅰ, Ⅲ, Ⅳ, and genotype Ⅳ was the most prevalent. All isolates had varying degrees of hemolytic activity. Except one case isolate, the remaining isolates had the ability to form biofilms in polystyrene surface. Genotype Ⅲ strains had stronger hemolytic activity and biofilm formation capacity.Conclusion s Ten Trichosporon asahii isolates from urinary tract infection were mainly genotype Ⅳ, while genotype Ⅲ strains showed stronger hemolytic activity and biofilm formation ability among all strains.

Key words: Trichosporon asahii, genotype, hemolysis, biofilm

CLC Number:

R379.9

SUN Wei, SU Jian-rong, XU shu-zhen. Studies on the relationship between hemolytic activity and biofilm formation ability of Trichosporon asahii isolates and their genotypes[J]. Chinese Journal of Mycology, 2013, 8(3): 147-150.

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