Deletion and reconstruction of HOG1 gene in Cryptococcus gattii

Abstract

Abstract: Objective To construct the HOG 1 mutant strain and the HOG 1 reconstituted strain.Methods Cloning HOG 1 gene according to the gene sequences, we got dHOG 1 gene by deleting part of HOG 1 gene.Then the HOG 1 gene and dHOG 1 gene were subcloned into eukaryotic vectors pGAPzα-A respectively. Finally, pGAPzα-dHOG 1 was transferred into Crypcococcus gattii original strain to get HOG 1 mutant strain. By the same method, pGAPzα-HOG 1 vector was transferred into HOG 1 mutant strain to get HOG 1 reconstituted strain.Results Results of RT-PCR showed that the HOG 1 mutant strain did not express whole HOG 1 gene fragment, while HOG 1 reconstituted strain expressed intact HOG 1 gene.Conclusion Successful construction of HOG 1 mutant strain and HOG 1 reconstituted strain would be useful in further research of virulence and pathogenesis of Cryptococcus gattii.

Key words: Cryptococcus gattii, HOG 1 gene, mutant, reconstituted strain

CLC Number:

R379.5

HUANG You-ming, LOU Yong-hua, ZHU Hong-mei, WEN mei. Deletion and reconstruction of HOG1 gene in Cryptococcus gattii[J]. Chinese Journal of Mycology, 2013, 8(3): 137-141.

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