Abstract: Objective To observe the effect of 1064nm Nd:YAG laser irradiation on the growth of 5 main pathogenic fungi of onychomycosis including Trichophyton rubrum, Trichophyton mentagrophytes, Candida albicans, Candida parapsilosis, Aspergillus fumigatus.Methods Suspension of T.rubrum,T.mentagrophytes,C.albicans,C.parapsilosis were spotted on ager media. After incubation for several days, the colonies were exposed to various energies of 1064nm Nd:YAG laser. For T.r and T.m, diameters of colonies were measured on the first, third, and sixth days after laser irradiation. For C.a and C.p, the subculture's survival rates of different energies of laser irradiation were assessed respectively. As for A.fumigatus, having been incubated on the whole plate, to compare the different diameters of circular growth-free zones after different energies of laser treatment.Result In vitro, when the laser energy reaching 3 200 J/cm², the growth of five pathogenic fungi of onychomycosis could be inhibited significantly. And the higher the laser energy was, the more significant the inhibition would be, and even pathogenic fungi would be killed.Conclusion When the energy of 1064nm Nd:YAG laser reaching a certain amount, the pathogenic fungi of onychomycosis could be inhibited significantly or be killed.

Key words: onychomycosis, dermatophytes, candida, nondermatophytes, 1064nm Nd：YAG laser