Abstract: Objective To construct the overexpression of SPE1 in Candida albicans.Methods We inserted SPE1 open reading frame (ORF) under the control of the MET3 promoter in pCaEXP plasmid to construct pCaEXP-SPE1 plasmids which can overexpress SPE1 gene. Then we use lithium acetate method to transform the pCaEXP-SPE1 into Candida albicans RM1000 and select positive colony in SD-ura^-met^-cys^-selective solid culture medium. PCR was used to investigate the integration and the level of mRNA of SPE1 gene was detected by Real Time RT-PCR.Results SPE1-overexprssing plasmid was exactly established by restriction enzyme digestion.SPE1-overexpressing strain was successfully constructed as confirmed by PCR. And we used Real Time RT-PCR to successfully select a SPE1-overexpressing strain.Conclusions A strain highly expressing SPE1 gene can be successfully constructed by using the pCaEXP plasmid and the gene homologous recombination.

Key words: Candida albicans, SPE1, overexpression