Comparison of in vitro activities of itraconazole and hydroxyl-itraconazole

Chinese Journal of Mycology　2013, Vol. 8 　Issue (1): 26-29,34.

Comparison of in vitro activities of itraconazole and hydroxyl-itraconazole

GUO Li-na¹, XU Ying-chun¹, CHU Yun-zhuo², LV Huo-xiang³, CHEN Dong-mei⁴, TONG Ming-qing⁵, LIU Yong⁶, XI Li-yan⁷, SHI Yi⁸, YU Yun-song⁹, ZHOU Xin¹⁰

1. Chinese Academy of Medical Sciences, Peking Union Medical College, Clinical Microbiology Laboratory, Peking Union Medical College Hospital, Peking 100730, China;
2. The First Hospital of China Medical University, Shenyang 110001, China;
3. Zhejiang Provincial People's Hospital, Hangzhou 310014, China;
4. The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China;
5. Jiangsu Province Hospital, Nanjing 210029, China;
6. Shengjing Hospital of China Medical University, Shenyang 110004, China;
7. The Second Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510120, China;
8. Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China;
9. The First Affiliated Hospital of Zhejiang University, Hangzhou 310003, China;
10. Shanghai First People's Hospital, Shanghai 200080, China

Received:2012-09-25 Online:2013-02-28 Published:2013-02-28

Abstract

Abstract: Objective The in vitro activities of itraconazole and hydroxyl-itraconazole against clinically important fungi isolates were tested and compared.Methods The yeast isolates were obtained from blood and normal sterile body fluids, and the Aspergillus isolates were from deep respiratory specimens. The yeast isolates were identified by CHROMagar medium, API 20C and microscopy for Aspergillus strains. Broth microdilution testing was carried out according to CLSI document exactly.Results From 10 hospitals, we collected 338 pathgentic fungi, including 281 strains of Candida spp, 37 strains of Aspergillus spp, 18 strains of Cryptococcus neoformans and 2 other yeasts isolates. Itraconazole exhibited good activities to most yeast isolates. The susceptible rate of Candida albicans,C.tropicallis,C.parapsilosis,C.krusei were 83.8%,70.8%,78.6%,38.5%, respectively;more than 50% C.krusei isolates were susceptible dose dependent; respectively. Itraconazole has less susceptibility to C.glabrata.To Cryptococcus neoformans,Aspergillus fumigatus,A.flavus, the MIC₅₀/MIC₉₀ were 0.5/0.5、1/1、1/1 μg/mL, respectively. The antifungal activity of H-ITZ is similar to ITZ with against isolates tested.Conclusion Itraconazole has good activity against most pathgentic fungi tested, resistant isolates increased among C.glabrata;the major metabolite of itraconazole has in vitro antifungal activity.

Key words: itraconazole, hydroxyl-itraconazole, antifungal activities, broth microdilution test

CLC Number:

R978.5

GUO Li-na, XU Ying-chun, CHU Yun-zhuo, LV Huo-xiang, CHEN Dong-mei, TONG Ming-qing, LIU Yong, XI Li-yan, SHI Yi, YU Yun-song, ZHOU Xin. Comparison of in vitro activities of itraconazole and hydroxyl-itraconazole[J]. Chinese Journal of Mycology, 2013, 8(1): 26-29,34.

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