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Chinese Journal of Mycology 2012, Vol.  Issue (4): 199-202.

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Construction of knockout plasmid of IPF14744 gene of Candida albicans

TAN Hong-yue1, LI Ping1, HU Chan1, ZHU Hong-mei1, GU Jun2, WEN Hai1   

  1. 1. Institute of dermatology and fungal disease, Key Laboratory of the army fungal disease, Department of dermatology, Changzheng Hospital, Shanghai 200003, China;
    2. Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2012-06-22 Online:2012-08-28 Published:2012-08-28

Abstract: Objective To construct the plasmid for IPF 14744 knockout in Candida albicans.Methods Up and down streams of IPF 14744 gene ORF were amplified and inserted into the p5921 plasmid by restriction enzymes.Results The knockout plasmid pUC-14744-URA3 was successfully constructed.Conclusions The knockout plasmid is helpful for homologous recombination knockout of IPF 14744 in Candida albicans.

Key words: Candida albicans, IPF 14744 gene, plasmid

CLC Number: