Construction of vector and knockout plasmid of YPD1 gene of Candida albicans

Chinese Journal of Mycology　2010, Vol. 5 　Issue (2): 97-100.

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Construction of vector and knockout plasmid of YPD1 gene of Candida albicans

CHEN Yu-chong^1,2, WEN Hai¹, GAO Ping-hui³, CHEN Jiang-han¹, GU Ju-lin¹, ZHENG Zhi-zhong ²

1. Department of Dermatology and Mycology Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China;
3. Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received:2009-11-09 Online:2010-04-28 Published:2010-04-28

Abstract

Abstract: Objective To construct the plasmid for YPD1 knockout in Candida albicans.Methods The gene YPD1 of Candida albicnas was cloned in pBluescript II/SK+.The marker gene URA3 from plasmid p5921 was inserted into the YPD1 by restriction enzyme.Results The vector plasmid pBluescript-YPD1 and knockout plasmid pBluescript-YPD1-URA3 were successfully constructed.Conclusions The plasmid pBluescript-YPD1-URA3 is helpful for homologous recombination knockout of YPD1 in Candida albicans.

Key words: Candida albicans, gene, plasmid

CLC Number:

R379.

CHEN Yu-chong, WEN Hai, GAO Ping-hui, CHEN Jiang-han, GU Ju-lin, ZHENG Zhi-zhong. Construction of vector and knockout plasmid of YPD1 gene of Candida albicans[J]. Chinese Journal of Mycology, 2010, 5(2): 97-100.

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Construction of vector and knockout plasmid of YPD1 gene of Candida albicans

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