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Chinese Journal of Mycology 2010, Vol. 5  Issue (3): 133-136.

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Experimental study on the gene expression of MCP-1 and MIP-2 in chromomycosis in mice

CHAI Bao1, XIONG Ying2   

  1. 1. Department of Dermatology, Shenzhen Nanshan Hospital, affiliated to Guangdong Medical College;
    2. Department of Dermatology, Maternal and Child Hospital of Shenzhen, affiliated to Southern Medical University Shenzhen, Shenzhen 518052, China
  • Received:2010-03-29 Online:2010-06-28 Published:2010-06-28

Abstract: Objective To detect the gene expression of chemokines in mice model of skin chromomycosis and explore their roles in protective mechanism of the host.Methods Active or inactive C.carrion suspension (1 × 108 cfu/mL,0.025mL) was inoculated subcutaneously in the feet pads of healthy or immunosuppressive mice,respectively.mRNAs of MCP-1 and MIP-2 in the leision were examined by a real-time fluorescent quantitative PCR (FQ-PCR),and protein levels of MCP-1 and MIP-2 in the lesion tissue were examined by ELISA on day 7,30 and 60 after inoculation.Results Expression of MCP-1 and MIP-2 in healthy mice inoculated with active C.carrion on day 7 was higher than that on other days or that in other groups on day 7.But there were no significant differences on day 30 or day 60.Conclusions Both MCP-1 and MIP-2 possiblely participated in the pathogenesis of chromomycosis model due to C.carrion.

Key words: chromomycosis, MCP-1, MIP-2

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