Candida in onychomycosis:strain indentification drug sensitivity analysis and relationship to genotype

Abstract

Abstract: Objective To study strain classification and drug sensitivity of Candida in onychomycosis,and to clusteringly analysize the genotypes of C.albican and C.parapsilosis which are differently sensitive to miconazole.Methods Drug sensitivity of Candida were examined with ROSCO antifungal paper.Genotype analysis of C.albican and C.parapsilosis sensitive,medisensitive or resistant to miconazole was performed by ERIC-PCR.Results Drug sensitivity rates of C.albican and C.parapsilosis were 42% and 32% respectively.No specific DNA profiles of C.albican and C.parapsilosis were identified.Conclusions ERIC-PCR method could be applied in genotype analysis for different strains of Candida.Genotype of Candida has no relationship with resistance to miconazole.

Key words: Candida, drug sensitivity, ERIC-PCR, genotype

CLC Number:

R379.4

CAO Yan-yun, XU Shun-ming. Candida in onychomycosis:strain indentification drug sensitivity analysis and relationship to genotype[J]. Chinese Journal of Mycology, 2010, 5(6): 347-350,354.

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